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为了提高SiO2载体材料的酶固定化性能，分别采用接枝法和原位法对自制的分级介孔SiO2空心微球进行氨基修饰，并考察其木瓜蛋白酶固定化性能。与未修饰的样品相比，接枝法修饰后微球一级最可几孔径(8.68～8.70 nm)减小，二级最可几孔径(29.30～29.89 nm)无明显变化，表明氨基主要修饰在一级孔道中。原位法修饰后样品的一级最可几孔径(4.47～7.34 nm)、二级最可几孔径(14.49～19.78 nm)均有明显减小，表明一、二级孔道中均有氨基修饰。与未修饰和接枝法修饰的样品相比，原位法修饰后样品的最大木瓜蛋白酶固定量为750 mg·g-1，相对酶活力为121.0%，均高于未修饰和接枝法修饰的样品。原位法氨基修饰在提高SiO2载体材料的酶固定化性能方面更具优势。
Abstract：In order to improve enzyme immobilization performance of SiO2 carrier materials,homemade hierarchical mesoporous SiO2 hollow microspheres were modified with amino groups by grafting and in-situ methods,respectively.Their application in papain immobilization was investigated.Compared with unmodified samples,primary most probable pores (8.68-8.70 nm) of the grafting modified microspheres decrease and secondary most probable pores (29.30-29.89 nm) do not change significantly,which indicates that the amino groups are mainly grafted in primary pores.Both primary (4.47-7.34 nm) and secondary most probable pores(14.49-19.78 nm) decrease significantly after modification via the in-situ method,which indicates that amino groups are modified in both areas.The maximum papain immobilization amount and its corresponding relative enzyme activity of the in-situ modified samples were 750 mg·g-1 and 121.0%,respectively,which were higher than those modified by grafting and unmodified samples.In-situ amino modification has advantages in improving enzyme immobilization performance of SiO2 carriers.
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基本信息:
中图分类号:O629.8
引用信息:
[1]李群艳,孙路瑶,韩寿德,等.分级介孔SiO_2空心微球氨基修饰及木瓜蛋白酶固定化[J],2022,36(05):708-714.
基金信息:
国家重点研发计划(2I009011201709)