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针对微生物法制备α-酮异己酸中目标物分离纯化困难的问题,以甲基丙烯酸羟乙酯和甲基丙烯酸丁酯为基质单体,采用冷冻结晶致孔和聚合法,制备半疏水基质晶胶,并接枝N,N,N-三甲基乙烯基苯甲氯化铵,得到半疏水基质阴离子交换晶胶,用于α-酮异己酸的层析分离。结果表明:在流速为1 cm·min-1和上样质量浓度为2.0 mg·m L-1的条件下,半疏水基质阴离子交换晶胶对牛血清白蛋白和α-酮异己酸的吸附容量分别达4.42和17.56 mg·m L-1,其对α-酮异己酸的吸附容量随NaCl浓度的增大而降低,且用浓度高于0.3 mol·L-1的NaCl溶液可实现洗脱。研究结果可为微生物法制备α-酮异己酸的分离材料选用提供参考。
Abstract：Semi-hydrophobic anion exchange cryogel was prepared by cryo-polymerization using 2-hydroxyethy methacrylate and butyl methacrylate as the key monomers via graft polymerization with monomer (vinylbenzyl) trimethylammonium chlorid,which was used for the separation ofα-ketoisocaproate prepared by biological methods.The results show that the binding capacity of the semi-hydrophobic cryogel for bovine serum albumin andα-ketoisocaproate were 4.42 and 17.56 mg·m L-1,respectively,when the loading concentration is 2.0mg·m L-1 at the flow velocity of 1 cm·min-1.The adsorption capacity forα-ketoisocaproate decreased with the increase of NaCl concentration and the elution was achieved by increasing NaCl concentration over 0.3 mol·L-1.The results of this work are helpful for the selection of materials forα-ketoisocaproate acid separation from biological preparation.
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基本信息:
中图分类号:TQ225;O647.3
引用信息:
[1]曲兴,楼小玲,张颂红,等.半疏水基质阴离子交换晶胶对α-酮异己酸的层析吸附特性[J],2022,36(01):46-52.
基金信息:
国家自然科学基金(22078296,21576240);; 浙江省自然科学基金(LD21B060001)
2021-07-20
2021
2021-12-10
2021
1