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造血干/祖细胞(hematopoietic stem/progenitor cells,HSPCs)体外扩增过程中，HSPCs的命运取决于HSPCs的对称或不对称分裂，而HSPCs分裂模式的选择与细胞因子有关。为此，以脐带血CD34+细胞为研究对象，采用SCF+TPO+FL(STF)、SCF+IL-3+IL-6(S36)和SCF+TPO+FL+IL-6(STF6)三种细胞因子组合进行体外扩增，分析了CD34+细胞扩增倍数、扩增后CD34+细胞的集落形成能力等HSPCs扩增特性；并以CD34~+CD48~-细胞的含量、Numb在子细胞中的分布以及numb和musashi-2的基因表达水平为评价指标，多角度评价细胞因子组合对HSPCs的分裂模式的影响。结果发现，与S36相比，STF和STF6均能够通过显著增加HSPCs自我更新的对称分裂比例提高HSPCs的体外扩增效果和集落形成能力。该研究结果可为HSPCs体外扩增过程的优化提供技术支持。
Abstract：During ex vivo expansion of hematopoietic stem/progenitor cells (HSPCs),the fate of HSPCs depends on symmetric or asymmetric division of HSPCs,and the choice of HSPCs division mode is related to cytokines.Therefore,cord blood CD34~+cells were used for ex vivo expansion by SCF+TPO+FL (STF),SCF+IL-3+IL-6 (S36) and SCF+TPO+FL+IL-6 (STF6) in this study.Expansion characteristics of HSPCs were analysed by CD34~+cell expansion folds and colony formation ability of expanded CD34~+cells and the proportion of CD34~+CD48~-cells,and the distribution of Numb in the daughter cells and the gene expression levels of numb and musashi-2 were used as evaluation indices to evaluate the effect of cytokine combination on the division mode of HSPCs from multiple perspectives.These results indicate that compared with S36,both STF and STF6 could significantly increase the ex vivo expansion and colony formation capacity of HSPCs by increasing self-renewal symmetric division.The results provide technical support for the optimization of HSPCs ex vivo expansion processes.
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中图分类号:Q253
引用信息:
[1]朱雪君,蔡海波,谭文松.细胞因子组合对体外扩增造血干/祖细胞分裂模式选择的影响[J],2022,36(05):702-707.