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针对扩张床吸附进料中含有较高浓度的生物质固体颗粒的特殊性,选择特殊离子(Li+和 Br-)作为停留时间分布(RTD)示踪物,系统考察了离子选择性电极的工作范围、响应时间、流速、生物质固体颗粒浓度的影响,证明了离子选择性电极的可用性。通过优化脉冲离子浓度和脉冲量,确定了合适的脉冲条件为 0.1mol?L?1和 0.5mL。以面包酵母为模型生物质、基因重组大肠杆菌匀浆液为实际对象,进行了含有固体颗粒进料时的 RTD 测定,考察不同生物质固体颗粒浓度和扩张床吸附剂的影响,不仅证实了所建立方法的可行性,而且是一种真实操作条件下扩张床稳定性评价的有效手段。
Abstract：Aiming at the particularity of expanded bed adsorption ?? high content of bio-particles in the feedstock, the special ions (Li+ and Br-) was chosen as the tracer for residence time distribution (RTD) measurement in the presence of bio-particles. Some factors were investigated to prove the suitability of the ion-selective electrode, such as working range, response time, flow rate and concentration of bio-particles. The conditions of injection pulse were studied, the ionic concentration of pulse and the amount of pulse were optimized and the suitable pulse conditions are 0.1 mol?L?1 and 0.5mL respectively. With the intact yeast cells as model bio-particles and E. coli homogenate as the real application object, RTD measurement was carried out in the presence of bio-particles, and the influences of bio-particles concentration and operation conditions were evaluated. The results demonstrate that the RTD analysis based on the ion-selective electrode is feasible and could be used as a good method for stability evaluation of expanded bed adsorption in the real application conditions.
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基本信息:
中图分类号:TQ028.8;TQ028.1
引用信息:
[1]吕淼华,林东强,赵文婷,姚善泾,朱自强.含有生物质固体颗粒进料时的扩张床稳定性评价[J],2004(06):751-755.
基金信息:
国家自然科学基金(20206029);;教育部留学回国人员科研启动基金