庄冰佳¹,于道永²,黄磊¹,徐志南¹,蔡谨¹

• 1:浙江大学 化学工程与生物工程学院，浙江 杭州 310027
• 2:中国石油大学(华东) 化学工程学院，山东 青岛 266580

摘要(Abstract)：

水通道蛋白Z （AQPZ）具有水选择专一性强、渗透性高等特点，可广泛用于制备水回收和脱盐的生物仿生膜。定点突变詹氏甲烷球菌（Methanococcus jannaschii）的酪氨酸-tRNA合成酶（tyrosyl-tRNA synthetase，TyrRS），使其可催化非天然氨基酸（丙炔氧基-L-苯丙氨酸（p-propargyloxyphenylalanine，pPpa））合成对应氨酰tRNA。将上述转运系统与大肠杆菌无细胞体系结合，在AQPZ的特定位点引入了pPpa将改性的AQPZ （P-AQPZ）重构到1，2-二油酰基磷脂酰胆碱（1，2-dioleoyl-sn-glycero-3-phosphocholine，DOPC），经停留光谱分析，其水渗透因子P_f值为3.42×10^-4 m·s^-1，是AQPZ的2.78倍。用截留反渗透装置分析发现P-AQPZ仿生膜的水通量高于AQPZ仿生膜并可保持较高的截盐率。

关键词(KeyWords)： 非天然氨基酸;水通道蛋白Z;基因编码;生物仿生膜;无细胞体系

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目（21276226，21306164）。

作者(Author): 庄冰佳¹,于道永²,黄磊¹,徐志南¹,蔡谨¹

DOI: 10.3969/j.issn.1003-9015.2016.00.039

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