丰诚杰¹,刘健¹,吴春雷¹,沈润溥¹,杨金纬²,杜奎¹

• 1:绍兴文理学院 化学化工学院，浙江 绍兴 312000
• 2:浙江昂利康制药股份有限公司，浙江 绍兴 312000

摘要(Abstract)：

为了对肿瘤细胞硫化氢（H₂S）进行定量检测，降低基于喹啉母环设计的H₂S荧光探针活细胞成像背景干扰，基于叠氮还原的H₂S荧光探针设计策略，以5-位炔烃取代喹啉替代5-三氮唑取代喹啉，提高探针本身的荧光量子产率，设计制备了一种新的基于喹啉母环的比率型H₂S荧光探针QL-N₃。结果表明，喹啉母环经5-炔烃修饰后，探针本身的荧光量子产率提高到0.12，表现出明显的比率型荧光探针特点。同时，QL-N₃探针保持对H₂S的高选择性、高检测灵敏度（检测极限为1.8×10^-8 mol·L^-1）和高检测速率（30 s），在HeLa细胞H₂S荧光成像中表现出较好效果。

关键词(KeyWords)： 硫化氢;荧光探针;比率型;快速响应;荧光成像

Abstract:

Keywords:

基金项目(Foundation): 浙江省公益基金项目（LGG19B02002）；绍兴文理学院博士研究启动基金（20185020）。

作者(Author): 丰诚杰¹,刘健¹,吴春雷¹,沈润溥¹,杨金纬²,杜奎¹

DOI: 10.3969/j.issn.1003-9015.2021.06.020

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