基于新型酶电极的微生物发酵多组分在线检测流路控制系统Multi-component online detection flow path control system for microbial fermentation based on new enzyme electrode
乔文杰,薄翠梅,蒋书波,王轶卿,李俊,储震宇,金万勤
摘要(Abstract)：
针对微生物发酵过程中多组分原位在线监测问题,基于普鲁士蓝(PB)纳米材料制备的新型酶生物传感电极,研究一种原位在线多通道检测流路控制系统。根据酶生物传感电极微电流响应特性,创新设计一种多通道泵阀协同作用流路控制系统,可有效降低交叉污染,提高设备可靠性。根据流路检测逻辑时序要求,研制多组分检测过程移液针精准定位、微量精确进样、定标检测等软件程序模块。采用细分驱动电路控制步进电机,结合梯形加减速算法实现移液针的精准定位和微量精确进样,通过自主调节进样量和定标检测模块,实现多组分、宽范围、快速精确检测。实验结果表明该系统可实现葡萄糖、赖氨酸和乳酸等多组分浓度的宽范围检测,检测精度在2%以内,能够较好地满足微生物发酵多组分在线检测需求。
关键词(KeyWords)： 酶生物传感电极;多组分在线检测;流路控制系统;加减速算法;微量精确进样
基金项目(Foundation): 国家自然科学基金(21727818,62173178);; 国家重点研发计划(2019YFB1705800)
作者(Author): 乔文杰,薄翠梅,蒋书波,王轶卿,李俊,储震宇,金万勤
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