吕惠生,周锦怡,耿中峰,李永辉,吕春柳,刘双彦

• 1:天津大学石油化工技术开发中心教育部绿色合成与转化重点实验室

摘要(Abstract)：

将超临界流体色谱技术应用于赤芍活性组分的分离。采用Zorbax SB-CN色谱柱(9.4 mm×250 mm,5μm),考察了夹带剂、CO_2流量、温度以及压力对组分分离度的影响,得到了适宜的分离条件:夹带剂含量13.64%(wt),CO_2流量20 g×min~(-1),柱温313.15 K,柱压12 MPa。此条件下制备了纯度为94.11%(wt)的芍药苷和85.65%(wt)的芍药内酯苷产品,收率分别为95.6%和88.3%。建立了容量因子模型,芍药苷和芍药内酯苷模型的平均相对偏差分别为1.20%和0.72%,拟合结果良好。

关键词(KeyWords)： 超临界流体色谱;制备;芍药苷;芍药内酯苷;容量因子

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 吕惠生,周锦怡,耿中峰,李永辉,吕春柳,刘双彦

参考文献(References)：

• [1]Zhou J,Wang J,Li W,et al.Paeoniflorin attenuates the neuroinflammatory response in a rat model of chronic constriction injury[J].Molecular Medicine Reports,2017,15(5):3179-3185.
• [2]Wei Y,La L,Wang L,et al.Paeoniflorin and liquiritin,two major constituents in Chinese herbal formulas used to treat hyperprolactinemia-associated disorders,inhibits prolactin secretion in prolactinoma cells by different mechanisms[J].Journal of Ethnopharmacology,2017,204(10):36-44.
• [3]SUN Rong(孙蓉),LüLi-li(吕丽莉),GUO Shou-dong(郭守东),et al.Effect of paeoniflorin on focal cerebral ischemia model and blood-brain barrier(芍药苷对局灶性脑缺血模型及血脑屏障的影响)[J].Journal of Harbin University of Commerce(Natural Sciences Edition)(哈尔滨商业大学学报(自然科学版)),2005,21(4):405-410.
• [4]Li W,Qi Z,Wei Z,et al.Paeoniflorin inhibits proliferation and induces apoptosis of human glioma cells via micro RNA-16upregulation and matrix metalloproteinase-9 downregulation[J].Molecular Medicine Reports,2015,12(2):2735-2740.
• [5]Wang Y L,Wang J X,Hu X X,et al.Antidepressant-like effects of albiflorin extracted from Radix paeoniae Alba[J].Journal of Ethnopharmacology,2016,179(9):9-15.
• [6]GUO Xian-shuai(郭先帅),LIU Dong-yun(刘冬云),ZHU Wan-ting(朱婉婷),et al.Purification technology of extracts from prunuspersica and paeonialactiflora in compound guizhifuling by AB-8 macroporous absorption resin(AB-8型大孔吸附树脂纯化复方桂枝茯苓丸中桃仁、赤芍提取物工艺)[J].Journal of Guangdong Pharmaceutical University(广东药学院学报),2015,31(2):167-170.
• [7]LI Qian(李芊).Extraction and separation of paeoniflorin&albiflorin from radix paeoniaerubra(从白芍中提取及分离芍药苷和芍药内酯苷)[D].Anshan(鞍山):University of Science and Technology Liaoning(辽宁科技大学),2012.
• [8]Li W,Wang J,Zhang J.Extracting albiflorin/paeoniflorin,involves adding alumina to paeony extract,eluting,collecting liquid,purifying liquid in active carbon column,eluting in silica gel column,collecting,filtering solution,and drying filtrate:US,102304159[P].2006-02-01.
• [9]Wang R,Peng X,Wang L,et al.Preparative purification of peoniflorin and albiflorin from peony rhizome using macroporous resin and medium-pressure liquid chromatography[J].Journal of Separation Science,2012,35(15):1985-1992.
• [10]Nie L,Dai Z,Ma S.Improved chiral separation of(R,S)-goitrin by SFC:an application in traditional chinese medicine[J].Journal of Analytical Methods in Chemistry,2016,2016(1):1-5.
• [11]Yang H,Ying F,Tang G,et al.Development and validation of a fast SFC method for the analysis of flavonoids in plant extracts[J].Journal of Pharmaceutical&Biomedical Analysis,2017,140(10):384-391.
• [12]DU Jin-bao(杜金宝),DENG Li-kang(邓立康),YAN Li(闫莉),et.al.Research progress on supercritical(超临界色谱技术制备功能性产品研究进展)[J].Cereal&Food Industry(粮食与食品工业),2013,20(4):26-30.
• [13]Miller L.Pharmaceutical purifications using preparative supercritical fluid chromatography[J].Chimica Oggi-Chemistry Today,2014,32(2):23-26.
• [14]LI Jia-xu(李嘉旭).The preparative separation of two kind of drugs by simulated moving bed chromatography(模拟移动床色谱分离福莫特罗对映体过程研究)[D].Shanghai(上海):East China University of Science and Technology(华东理工大学),2016.
• [15]LüHui-sheng(吕惠生),WANG Zhi(王智),GENG Zhong-feng(耿中峰),et.al.Measurements and prediction of infinite diffusion coefficients for paeoniflorin and albiflorin in supercritical carbon dioxide(芍药苷及芍药内酯苷在超临界CO2中无限稀释扩散系数的测定与计算)[J].Journal of Chemical Engineering of Chinese Universities(高校化学工程学报),2018,32(1):16-23.
• [16]HE Yan(何岩),ZHANG Min-hua(张敏华),DONG Xiu-qin(董秀芹).Molecular dynamics simulation for calculating the diffusion coefficients of acetone,ethyl acetate and cyclohexane at infinite dilution solution of supercritical CO2(常用夹带剂在超临界CO2中无限稀释扩散系数的MD计算)[J].Journal of Chemical Engineering of Chinese Universities(高校化学工程学报),2008,22(1):152-156.
• [17]PANG Fei(庞斐),LüHui-sheng(吕惠生),LIU Yan(刘岩),et.al.The study on purification of artemisinin by supercritical fluid chromatography(超临界流体色谱纯化Z-藁本内酯的研究)[J].Journal of Chemical Engineering of Chinese Universities(高校化学工程学报),2010,24(4):569-573.
• [18]MO Xu-fei(莫绪飞),LüHui-sheng(吕惠生),ZHANG Min-hua(张敏华),et al.The study on purification of Z-ligustilide by supercritical fluid chromatography(超临界流体色谱纯化青蒿素的研究)[J].Journal of Chemical Engineering of Chinese Universities(高校化学工程学报),2013,27(5):737-742.
• [19]Bolten D,Johannsen M.Influence of 2-propanol on adsorption equilibria ofα-andδ-tocopherol from supercritical carbon dioxide on silica gel[J].Journal of Chemical&Engineering Data,2006,51(6):2132-2137.