俞明,钱俊青,龚峰,唐弋奥

• 1:浙江工业大学 药学院，浙江 杭州 310014

摘要(Abstract)：

将生物印迹和固定化酶方法结合，简化了印迹酶的制备流程，优化了固定化印迹脂肪酶的制备条件，筛选了最优印迹分子橄榄油，探讨了印迹最适pH值、印迹分子橄榄油的用量、印迹时间和载体离子型树脂的种类等影响因素对固定化印迹脂肪酶酯化效果的影响，得到了其制备的最佳工艺：印迹pH值8.0，橄榄油100 mg，1 mL乙醇为助溶剂，100 mg吐温20为表面活性剂，加入2 g 214型离子交换树脂，印迹时间为20 min。此工艺条件下制备得到的固定化印迹脂肪酶在反应中的酯化效果最高，固定化印迹酶的酯化效果是游离酶的4.35倍，固定化印迹酶催化酯化反应合成L-抗坏血酸棕榈酸酯后，最大产物浓度约为15.58 g·L^-1，最佳转化率可达63.3%。

关键词(KeyWords)： 脂肪酶;生物印迹;固定化;酯化反应;L-抗坏血酸棕榈酸酯

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划课题（2016YFD0400803）。

作者(Author): 俞明,钱俊青,龚峰,唐弋奥

DOI: 10.3969/j.issn.1003-9015.2017.04.033

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