高效利用内醚糖微生物的筛选鉴定及预处理对菌株利用热解液的影响Isolation and Identification of Efficient Levoglucosan-Assimilating Microorganisms and Effects of Pretreatment on their Utilization of Pyrolysis Liquids
孙欢,李东晓,陈育如,赵乙萱,刘军利,卫民
摘要(Abstract):
以纤维素热解产物内醚糖为碳源筛选到4株高效利用内醚糖的微生物,经ITS测序和菌落形态特征鉴定,4株菌分别为嗜松青霉(Penicillium pinophilum)、桔青霉(Penicillium citrinum)、冠突散囊菌(Eurotium cristatum)和红假单胞菌(Rhodopseudomonas sp)。对各菌株利用内醚糖的研究结果表明,嗜松青霉和桔青霉5 d内对2%内醚糖的利用率为100%,冠突散囊菌7 d内对2%内醚糖的利用率为100%,红假单胞菌在10 d对2%内醚糖的利用率为52%。将4株菌作用于热解液发现,红假单胞菌能在未经预处理的热解液中生长,14 d后对热解液中内醚糖的利用率为69%,另外3株菌能在经5%活性炭+Ca(OH)2预处理后的热解液中生长。对预处理后热解液成分的分析表明,预处理对热解液成分有显著影响,其中六甲基环三氧硅烷、1,2-环戊二酮、5-甲基糠醛、3-甲基-1,2-环戊二酮和羟甲基呋喃酮的去除率达100%,内醚糖的损失为27.1%。
关键词(KeyWords): 内醚糖;热解液;嗜松青霉;桔青霉;红假单胞菌
基金项目(Foundation): 江苏省高校自然科学研究重大项目(15KJA210002);; 江苏省“六大人才高峰”人才培养项目(NY-011-2015)
作者(Author): 孙欢,李东晓,陈育如,赵乙萱,刘军利,卫民
参考文献(References):
- [1]ZHANG Hong-xun(张洪勋),DAI Jiang-hong(代江红),WANG Xiao-yan(王晓燕),et al.A method of promotion the growth of pichia stipitis and production ethanol by levoglucosan(一种促进树干毕赤酵母的生长和发酵产乙醇的方法):CN,101 531 989 A[P].2009-04-22.
- [2]XIE Qing-ruo(谢清若),TONG Zhang-fa(童张法),WEI Teng-you(韦藤幼),et al.Optimization of bamboo pyrolysis process to prepare bio-oil by response surface methodology(响应曲面法优化竹材热裂解制备生物油的工艺研究)[J].J Chem Eng of Chinese Univ(高校化学工程学报),2012,26(3):487-492.
- [3]FAN Yong-sheng(樊永胜),CAI Yi-xi(蔡忆昔),LI Xiao-hua(李小华),et al.Bio-oil analysis and optimization of bio-oil yield from vacuum pyrolysis of rape straw using response surface methodology(响应面法优化油菜秸秆真空热解液化工艺及生物油分析)[J].J Chem Eng of Chinese Univ(高校化学工程学报),2015,29(3):628-633.
- [4]Giannoni M,Martellini T,Del Bubba M,et al.The use of levoglucosan for tracing biomass burning in PM2.5 samples in Tuscany(Italy)[J].Environmental Pollution,2012,167:7-15.
- [5]CHANG Dong-dong(常冬冬),YU Zhi-sheng(余志晟),ZHANG Hong-xun(张洪勋).Progress on pyrolysis of cellulose and its conversion to ethanol(纤维素热解液及其产物转化乙醇的研究进展)[J].Chinese Agricultural Science Bulletin(中国农学通报),2012,28(26):183-189.
- [6]REN Pu-xian(任普鲜),JIANG Jian-chun(蒋剑春),YANG Xiu-shan(杨秀山),et al.Research progress on production of ethanol as fuel from fast pyrolysis products of lignocellulose(木质纤维素快速热解产物生产燃料乙醇研究进展)[J].Biomass Chemical Engineering(生物质化学工程),2009,43(3):47-51.
- [7]LU Qiang(陆强),ZHU Xi-feng(朱锡峰).Production of levoglucosenone from pyrolysis of cellulose catalyzed by solid superacids(利用固体超强酸催化热解纤维素制备左旋葡萄糖酮)[J].Journal of Fuel Chemistry and Technology(燃料化学学报),2011,39(6):425-431.
- [8]DSTAG Radlein,A Grinshpun,J Piskorz,et al.On the presence of anhydro-oligosaccharides in the sirups from the fast pyrolysis of cellulose[J].Journal of Analytical and Applied Pyrolysis,1987,12(1):39-49.
- [9]Scott D S,Piskorz J,Radlein D.Yield of chemicals from biomass-based fast pyrolysis oils[J].Energy Biomass Wastes,1993,16:797-809.
- [10]Lede J,Panagopoulos J,Li H Z,et al.Fast pyrolysis of wood:direct measurement and study of ablation rate[J].Fuel,1985,64(11):1514-1520.
- [11]Bridgwater A V,Boocock D G B.Developments in thermochemical biomass conversion[M].London:Blackie Academic and Professional.1996.611-621.
- [12]Bennett N M,Helle S S,Duff S J B.Extraction and hydrolysis of levoglucosan from pyrolysis oil[J].Bioresource Technology,2009,100(23):6059-6063.
- [13]Mullen C A,Boateng A A,Goldberg N,et al.Bio-oil and bio-char production from corn cobs and stover by fast pyrolysis[J].Biomass and Bioenergy,2010,34(1):67-74.
- [14]Nakagawa M,Sakai Y,Yasui T.Itaconic acid fermentation of levoglucosan[J].Journal of Fermentation Technology,1984,62(2):201-203.
- [15]Prosen E M,Radlein D,Piskorz J,et al.Microbial utilization of levoglucosan in wood pyrolysate as a carbon and energy source[J].Biotechnology Bioengineering,1993,42(4):538-541.
- [16]Zhuang X L,Zhang H X,Yang J Z,et al.Preparation of Levoglucosan by pyrolysis of cellulose and its citric acid fermentation[J].Bioresource Technology,2001,79(1):63-66.
- [17]YU Zhi-sheng(余志晟),NING Bo-ying(宁洎英),ZHANG Ling(张玲),et al.Microbial investigation on assimilation and ethanol fermentation of levoglucosan from cellulosic pyrolysis products(纤维素热解产物内醚糖的微生物同化与乙醇发酵测试)[J].Chinese Agricultural Science Bulletin(中国农学通报),2011,27(6):342-349.
- [19]WU Meng-yao(吴梦瑶),HU Xiang(胡翔).Isolation and identification of a strain of 1-hexyl-3-methylimidazolium hexafluorophosphatedegrading bacterium(一株降解1-己基-3-甲基咪唑六氟磷酸盐微生物的筛选与鉴定)[J].J Chem Eng of Chinese Univ(高校化学工程学报),2014,28(6):431-437.