许青青^1,2,蒋越梅¹,张宏杰¹,汪艳¹,吕亮¹,杨亦文²,任其龙²

• 1:衢州学院 化学与材料工程学院，浙江 衢州 324000
• 2:浙江大学 生物质化工教育部重点实验室，浙江 杭州 310027

摘要(Abstract)：

植物甾醇的分子结构都很相似，但是生物活性相差很大，分离难度也很大。研究使用COSMO-RS（真实溶剂似导体屏蔽模型）预测，探讨离子液体对植物甾醇的萃取分离机理。预测结果表明，植物甾醇在离子液体中的溶解度随氢键相互作用的增强而提高，氢键碱性较强的离子液体将增加萃取容量；萃取剂极性增大，易与豆甾醇形成萃取相分离。实验筛选以离子液体[bmim]Cl为代表，构建了液-液两相体系，结合高效液相色谱分析测定离子液体复合萃取剂对植物甾醇的分配系数和分离选择性。实验结果表明：用[bmim]Cl-乙腈/正己烷两相体系萃取，随离子液体浓度的增加，分配系数和萃取选择性系数的增加十分显著，当用20%[bmim]Cl-乙腈萃取时，豆甾醇的分配系数可达7.89，豆甾醇对β-谷甾醇的分离选择性可达65.8，是纯乙腈为萃取剂时的54倍以上。

关键词(KeyWords)： 豆甾醇;β-谷甾醇;离子液体;COSMO-RS;萃取

Abstract:

Keywords:

基金项目(Foundation): 浙江省科技公益项目（2017C31124）；衢州市科技计划项目（2015Y001）；生物质化工教育部重点实验室基金（2015BCE004）；衢州市115人才工程培养人员（2014-2019）；国家自然科学基金（21476127，U1607119）。

作者(Author): 许青青^1,2,蒋越梅¹,张宏杰¹,汪艳¹,吕亮¹,杨亦文²,任其龙²

DOI: 10.3969/j.issn.1003-9015.2018.06.019

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