覃益民,苏加坤,唐江涛,王机,姚评佳,魏远安

• 1:广西大学化学化工学院
• 2:广西大学化学化工学院
• 3:广西大学化学化工学院
• 4:广西大学化学化工学院

摘要(Abstract)：

通过热变性、硫酸铵分级沉淀、DEAE-Sephadex A-50(柱1)、Octyl-Sepharose CL-4B、Sephacryl S-300、DEAE-Sephadex A-50(柱2)层析等分离纯化步骤对本实验室自行筛选诱变的米曲霉GX0011来源的β-果糖基转移酶进行了分离纯化,并通过SDS-PAGE检测了该酶的纯度和亚基分子量。结果表明电泳一条带,亚基表观分子量为5.1×104,小于多数文献报道的结果。纯化倍数为121.7,比活和活力回收分别为1975U-mg-1和17%。酶活测定表明该酶只起到转果糖基的作用而无水解活性。

关键词(KeyWords)： 低聚果糖;米曲霉;β-果糖基转移酶;分离纯化

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(29772096);; 广西高校博士点建设资金资助

作者(Author): 覃益民,苏加坤,唐江涛,王机,姚评佳,魏远安

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