壳聚糖酶的分离提纯及其酶学性质研究Purification and Properties of Chitosanase from Penicillium sp.ZD-Z1
隋斯光,方文建,郑连英
摘要(Abstract)：
从土壤中筛选获得的高产壳聚糖酶菌株Penicillium sp.ZD-Z1,经发酵、分离提取出两种壳聚糖酶A和酶B,并对它们分别进行酶学性质测试。分别测定酶A和酶B的等电点、相对分子质量、最适反应温度和pH、不同脱乙酰度的壳聚糖对酶活的影响。并考察了两种壳聚糖酶的酶解方式,结果表明,酶A为内切酶,酶B为外切酶。
关键词(KeyWords)： 壳聚糖酶;Penicillium sp.;分离纯化;酶学性质
基金项目(Foundation): 浙江省重点科技项目(2005c-22001);; 国家自然科重点基金资助项目(50233020)
作者(Author): 隋斯光,方文建,郑连英
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