薛学东,李伟,李志敏,周利,夏月兰,叶勤

• 1:华东理工大学生物反应器工程国家重点实验室

摘要(Abstract)：

在肺炎克雷伯杆菌厌氧发酵甘油生产1,3-丙二醇的过程中,大量的副产物,特别是乳酸和乙醇等副产物的生成,导致1,3-丙二醇的浓度和得率下降。为了进一步了解有机酸加入后菌体的代谢情况,在菌体生长期同时加入柠檬酸、琥珀酸和延胡索酸三种有机酸的混合液,使其浓度达到3.8 mmol·L-1。5 L罐发酵结果显示,产物的生成发生显著变化。同时,发酵动力学分析表明,有机酸加入后,甘油的比消耗速率和1,3-丙二醇的比生成速率显著提高,而乳酸和乙醇的比生成速率则明显下降。进一步对甘油代谢过程中的碳流分配分析表明,有机酸加入后,甘油进入还原途径的碳流比例由0.45显著增加为0.62,氧化途径的碳流比例由0.53减为0.35。

关键词(KeyWords)： 有机酸;肺炎克雷伯杆菌;厌氧发酵;甘油

Abstract:

Keywords:

基金项目(Foundation): 江苏省科技计划(BG2006036)资助项目;; 国家重点实验室专项经费(2060204)资助项目;; 上海市重点学科项目(B505)资助

作者(Author): 薛学东,李伟,李志敏,周利,夏月兰,叶勤

参考文献(References)：

• [1]Zeng A P,Biebl H.Bulk chemicals from biotechnology:the case of 1,3-propanediol production and the new trends[J].Adv BiochemEng/Biotechnol,2002,74:239-259.
• [2]Chuah H H,Brown H S,Dalton P A.Corterra polytrimethylene terephthalate-a new performance carpet fible[J].Int Fiber J,1995,10(5):50-52.
• [3]Alper J.Engineering metabolism from commercial gains[J].Science,1999,283(5408):1625-1626.
• [4]LüDan(吕丹),DU Wei(杜伟),LIU De-hua(刘德华).Study on soluble lipase NS81006-mediated alcohoysis of oils containing FFAfor biodiesel production(游离脂肪酶NS81006催化含酸油脂制备生物柴油的应用研究)[J].J Chem Eng of Chinese Univ(高校化学工程学报),2010,24(1):82-86.
• [5]Cheng K K,Zhang J A,Liu D H,et al.Pilot-scale production of 1,3-propanediol using Klebsiella pneumoniae[J].Process Biochem,2007,42(4):740-744.
• [6]Menzel K,Zeng A P,Deckwer W D.High concentration and productivity of 1,3-propanediol from continuous fermentation ofglycerol by Klebsiella pneumoniae[J].Enzyme Microb Technol,1997,20(2):82-86.
• [7]Menzel K,Zeng A P,Biebl H,et al.Kinetic,dynamic,and pathway studies of glycerol metabolism by Klebsiella plleumoniae inanaerobic continuous culture:I.The phenomena and characterization of oscillation and hysteresis[J].Biotechnol Bioeng,1996,52(5):549-560.
• [8]Zeng A P,Menzel K,Deckwer W D.Kinetic,dynamic,and pathway studies of glycerol metabolism by Klebsiella pneumoniae inanaerobic continuous culture:II.Analysis of metabolic rates and pathways under oscillation and steady-state conditions[J].Biotechnol Bioeng.1996,52(5):561-571.
• [9]Ahrens K,Zeng A P,Deckwer W D,et al.Kinetic,dynamic,and pathway studies of glycerol metabolism by Klebsiella pneumoniaein anaerobic continuous culture:III.Enzymes and fluxes of glycerol dissimilation and 1,3-propanediol formation[J].BiotechnolBioeng,1998,59(5):544-552.
• [10]Biebl H,Menzel K,Zeng A P,et al.Microbial production of 1,3-propanediol[J].Appl Microbiol Biotechnol,1999,52(3):289-297
• [11]Zeng A P,Biebl H,Schlieker H,et al.Pathway analysis of glycerol fermentation by Klebsiella pneumoniae:regulation of reducingequivalent balance and product formation[J].Enzyme Microb Technol,1993,15(9):770-779.
• [12]Chen K K,Liu H J,Liu D H.Multiple growth inhibition of Klebsiella pneumoniae in 1,3-propanediol fermentation[J].BiotechnolLett,2005,27(1):19-22.
• [13]Xue X D,Li W,Li Z M,et al.Enhanced 1,3-propanediol production by supply of organic acids and repeated fed-batch culture[J].JInd Microbiol Biotechnol,2010,37(7):681-687.
• [14]Lin R H,Liu H J,Hao J,,et al.Enhancement of PDO production by Klebsiella pneumoniae with fumarate addition[J].BiotechnolLett,2005,27(22):1755-1759.
• [15]Chen X,Xiu ZL,Wang JF,et al.Stoichiometric analysis and experimental investigation of glycerol bioconversion to 1,3-propanediolby Klebsiella pneumoniae under microaerobic conditions[J].Enzyme Micro Technol,2003,33(7):386-394.
• [16]Bradford P,Pohle W D,Gunther J K,et al.Determination of glycerol by oxidation with periodic acid[J].Oil&Soap,1942,19(11):189-193.
• [17]Senior A.ATP synthesis by oxidative phosphorylation[J].Physiol Rev,1988,68(1):177-231