徐宝多,徐志南,何宁,王颖,陈杰,李清彪,卢英华

• 1:厦门大学化学工程与生物工程系
• 2:浙江大学化学工程与生物工程系
• 3:厦门大学化学工程与生物工程系
• 4:厦门大学化学工程与生物工程系

摘要(Abstract)：

首先研究了应用于液芯羧甲基纤维素钠-海藻酸钙(CMC-ALG)微胶囊制备中的各种化学组分对重组盘基网柄菌生长的影响,然后考察不同组分浓度制备而成的各种CMC-ALG微胶囊内重组盘基网柄菌的生长情况,从而得到较适的微胶囊制备的组分配比(CMC12g·L-1,SA8g·L-1,CaCl2100g·L-1)。结果表明,在以上较适条件下制备的微胶囊内重组盘基网柄菌的生长得到了极大的改善,最大的细胞密度比游离培养时提高了4倍,达到了8.0×107mL-1;相应的人类可溶性Fas配体(FasL)的表达水平也提高了1.5倍,达到了315μg·L-1。最后,开展了微胶囊化重组盘基网柄菌的二次重复发酵FasL的研究,结果表明,经过二次重复批次培养,最大细胞密度可达到1.24×108mL-1,为游离培养的8～10倍,而且FasL的表达水平还能维持高水平(280μg·L-1),为游离培养时的2倍。

关键词(KeyWords)： CMC-ALG微胶囊;微囊化培养;盘基网柄菌;人类可溶性Fas配体

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助(20306025,30370039)

作者(Author): 徐宝多,徐志南,何宁,王颖,陈杰,李清彪,卢英华

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