PVA对生物合成细菌纤维素膜拉伸性能的影响The Effect of Polyvinyl Alcohol on Tensile Property of Biosynthesized Bacterial Cellulose Membrane
朱会霞,贾士儒,沈惠玲,杨洪江,贾原媛,张桂才
摘要(Abstract):
BC膜生物合成的过程受培养基中加入不同浓度PVA的影响,会产生拉伸性能各异的BC膜。培养基中添加低浓度(20和40 g·L·1)聚乙烯醇(PVA)后BC膜产量和拉伸强度等指标均有提高,培养至96 h时20和40 g·L·1 PVA浓度的培养基中BC产量分别比对照组高出40.48%和16.9%,此时40 g·L·1 PVA培养基中BC膜的弹性模量和拉伸强度最大,分别为4.56 GPa和66.8 MPa;而添加高浓度(60和80 g·L·1)PVA后BC膜产量和拉伸强度均降低。X-射线衍射结果显示培养基中低浓度的PVA可以提高膜的结晶度,而高浓度的PVA降低BC膜的结晶度;扫描电镜照片显示培养基中添加低浓度PVA后形成的BC膜结构更为致密均匀。
关键词(KeyWords): 聚乙烯醇;细菌纤维素膜;生物合成;弹性模量;拉伸性能
基金项目(Foundation): 国家自然基金委员会资助项目(20976133);; 天津科技大学优秀博士论文培育基金(B201002)
作者(Author): 朱会霞,贾士儒,沈惠玲,杨洪江,贾原媛,张桂才
参考文献(References):
- [1]Wan Y Z,Hong L,Jia S R,et al.Synthesis and characterization of hydroxyap atite–bacterial cellulose nanocomposites[J].ComposSci Technol,2006,66(11-12):1825-1832.
- [2]Yoshiaki Y,Shin K,Kenji T.Structural characterization of the Acetobacter xylinum endo-β-1,4-Glucanase CMCax required forcellulose biosynthesis[J].Proteins,2006,64(4):1069-1077.
- [3]Zhou LL,Sun DP,Hu LY.Effect of addition of sodium alginate on bacterial cellulose production by Acetobacter xylinum[J].J IndMicrobiol Biot,2007,34(7):483-489.
- [4]Keshk S.Physical properties of bacterial cellulose sheets produced in presence of lignosulfonate[J].Enzyme Microb Tech,2006,40(1):9-12.
- [5]Yamanaka S,Ishihara M,Sugiyama J.Structural modification of bacterial cellulose[J].Cellulose,2000,7(3):213-225.
- [6]Tokoh C,Takabe K,Sugiyama J,et al.CP/MAS 13C-NMR and electron diffraction study of bacterial cellulose structure affected bycell wall polysaccharides[J].Cellulose,2002,9(3-4):351-360.
- [7]TONG Zhi-ming(仝志明),LI Yan(李焱),CAO Zhu-an(曹竹安).Growth-promoting effect of polymers on hybridoma cells(高分子聚合物对杂交瘤细胞生长的促进作用)[J].Journal of Tsinghua University(Science and Technology)(清华大学学报(自然科学版)),1999,39(12):32-37.
- [8]LI Min(李敏),DU Guo-cheng(堵国成),CHEN Jian(陈坚).Biodegredation of polyvinyl alcohol(生物降解聚乙烯醇研究进展)[J].Journal of Food Science and Biotechnology(食品与生物技术学报),2008,27(5):8-12.
- [9]CAI Bang-xiao(蔡邦肖),FENG Wen-jie(冯文捷).Characteristics and preparation of PA/PVA/PA multilayer compositemembrane with alternate separation layers(PA/PVA/PA多层交替复合分离膜的制备与表征)[J].J Chem Eng of ChineseUniv(高校化学工程学报),2009,23(6):1030-1037.
- [10]ZHANG Chun-hua(仉春华),YANG Feng-1in(杨凤林),WANG Wen-jun(王文君),et al.Preparation of PVA modified non wovenfabric and research of its antifouling performance(聚乙烯醇改性无纺布的制备及耐污染性能的研究)[J].J Chem Eng of ChineseUniv(高校化学工程学报),2008,22(1):172-176.
- [11]SONG Zhao-xia(宋朝霞),ZHANG Ying(张颖),DU Guo-cheng(堵国成),et al.Optimization of fermentation conditions for aPVA-degrading enzyme producing strain(一株产聚乙烯醇降解酶的紫色杆菌的发酵条件研究)[J].J Chem Eng of ChineseUniv(高校化学工程学报),2006,20(5):769-774.
- [12]TANG Wei-hua(汤卫华).Biosynthesis of Bacterial Cellulose,its Property Analysis and Applications(细菌纤维素的生物合成、性质分析及其应用)[D].Tianjin(天津):Tianjin University of Science and Technology(天津科技大学),2009.
- [13]Tang Weihua,Jia Shiru,Jia Yuanyuan,et al.The influence of fermentation conditions and post-treatment methods on porosity ofbacterial cellulose membrane[J].World J Microbiol Biotechnol,2010,26(1):125-131.
- [14]TANG Wei-hua(汤卫华),MA Xia(马霞),JIA Yuan-yuan(贾原媛),et al.Bacterial cellulose production by Acetobacter xylinum instatic fed-batch culture(木醋杆菌流加静置培养生产细菌纤维素)[J].Food and Fermentation Industries(食品与发酵工业),2008,34(2):21-23.
- [15]Shiru Jia,Hongyu Ou,Guibing Chen,et al.Cellulose production from Gluconobacter oxydans TQ-B2[J].Biotechnol Bioproc E,2004,9(3):166-170.
- [16]Sarkar D,Yabusaki M,Hasebe Y,et al.Fermentation and metabolic characteristics of Gluconacetobacter oboediens for differentcarbon sources[J].Appl Microbiol Biotechnol,2010,27(2):127-136.
- [17]LIU Xue-qing(刘学清),LIU Ji-yan(刘继延).Preparation and characterization of chitosan/polyvinyl alcohol gradient material(壳聚糖/聚乙烯醇梯度材料的制备及表征)[J].China Plastics Industry(塑料工业),2008,36(10):20-23.