张雪洪,胡洪波,唐涌濂,黄瑞珊,陈正杰

• 1:上海交通大学生命科学技术学院
• 2:上海交通大学生命科学技术学院
• 3:上海交通大学生命科学技术学院
• 4:上海交通大学生命科学技术学院

摘要(Abstract)：

产物灵芝多糖在间歇深层发酵稳定期存在浓度下降现象。采用膜生物反应器系统能够及时分离产物,控制产物的积累,有效防止灵芝多糖的消耗。而灵芝菌丝体在反应器中不断循环,浓度得到进一步提高,并不断分泌灵芝胞外多糖,可延长产多糖时间,从而提高了胞外多糖的总产量。研究了不同稀释率0~0.24hr-1下连续发酵的多糖生成和菌丝体生长状况; 连续发酵时发酵液中灵芝多糖的浓度比间歇发酵要低,并随稀释率的增大而下降,菌丝体浓度则随稀释率的增大而增大。总的菌丝体干重可比间歇发酵增加20%以上,多糖总产量可达到间歇发酵的5倍以上。连续发酵也有利于灵芝多糖与菌丝体的分离。结果表明利用膜生物反应器进行液体连续发酵是生产和分离胞外灵芝多糖的一条有效途径, 并可为其它真菌胞外多糖的深层发酵生产提供借鉴。

关键词(KeyWords)： 灵芝多糖;深层发酵;膜生物反应器

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张雪洪,胡洪波,唐涌濂,黄瑞珊,陈正杰

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