专一性降解含硫杂环化合物菌株的分离和筛选Isolation of Bacteria Species for Degrading Sulfur Heterocyclics
张英,李伟,王妙冬,施耀
摘要(Abstract)：
从不同污泥与堆煤土样中分离出七株菌株,均能专一地切断二苯并噻吩(DBT)中的C-S键,沿4S途径代谢,生成2-羟基联苯(2-HBP)。通过对七株菌株46h休止细胞和繁殖期生长细胞脱硫活性的比较,筛选出具有较高脱硫活力的微杆菌(Microbacteriumsp.)ZD-M2和棒杆菌(Corynebacteriumsp.)ZD-C7,并在生长温度30℃,pH7.0,0.5mmol?L?1DBT作为唯一硫源,以甘油为碳源,用摇瓶培养法测定了它们的生长和脱硫曲线。以正十六烷为模型油相,在油水体积比为1.0的两相介质中考察两菌株的脱硫特性。结果表明,有机相的存在增加了菌株的脱硫活性,但活性持续时间只有9小时较水溶液介质中的短。同时两菌都能脱除燃料油中的其他含硫杂环化合物:噻吩、苯并噻吩、二苯硫醚和4,6-二甲基二苯并噻吩的硫,是有工业化应用前景的生物脱硫催化剂。
关键词(KeyWords)： 含硫杂环化合物;生物脱硫;分离;菌株
基金项目(Foundation): 国家自然科学基金(20276068)
作者(Author): 张英,李伟,王妙冬,施耀
参考文献(References)：
- [1]HUANG Xing-liang(黄星亮),SONG Yue-qin(宋月芹),SHEN Shi-kong(沈师孔),Studies on the action of promoters in Pd/Al2O3catalyst for liquid-phase hydrogenation of cracking gasoline(Pd/Al2O3液相选择加氢催化剂抗硫性能研究)[J].JChem Eng of Chinese Univ(高校化学工程学报),2002,16(5):575-579.
- [2]Kilbane J J.Desulfurization of coal:the microbial solution[J].Trends in Biotechnol,1989,7:97-101.
- [3]Sung-Keun R,Je H C,Young K C,et al.Desulfurization of dibenzothiophene and diesel oils by a newly isolated Gordona strain CYSK1[J].Appl Environ Microbiol,1998,64:2327-2331.
- [4]Kilbane J J.Mutant microorganisms useful for cleavage of organic C-S bonds[P].US Patent,5:104-801,1992.
- [5]Omori T,Monna L,Saiki Y,Kodama T.Desulfurization of dibenzothiophene by Corynebacterium sp.strain SY1[J].Appl Environ Microbiol,1992,58:911-915.
- [6]Izumi Y,Ohshiro T,Ogino H,et al.Selective desulfuization of dibenzothiophene by Rhodococcus erythropolis D-1[J].Appl Environ Microbiol,1994,60:223-226.
- [7]Furuya T,Kirimura K,Kino K,et al.Thermophilic biodesulfurization of dibenzothiophene and its derivatives by Mycobacterium phlei WU-F1[J].FEMS Microbiology Letters,2001,204:129-133.
- [8]WANG Miao-dong,LI Wei,SHI Yao,et al.The behavior of desulfurization of dibenzothiophene by Corynebacterium sp.ZD-1in aqueous phase[A].Proceedings of the Ener Env’2003[C].Changsha,China:Energy and Environment,October11-14,2003,513-519.
- [9]Ohshiro T,Suzuki K,Izumi Y.Regulation of dibenzothiophene degrading enzyme of Rhodococcus erythropolis D-1[J].Ferment Bioeng,1996,81:121-124.
- [10]Ohshiro T,Hirata T,Izumi Y.Microbial desulfurization of dibenzothiophene in the presence of hydrocarbon[J].Appl Microbiol Biotechnol,1995,44:249-252.
- [11]Patel S B,Kilbane J J,Webster D A.Biodesulphurisation of dibenzothiophene in hydrophobic media by Rhodococcus sp.Strain IGTS8[J].J Chem Technol Biotechnol,1997,69:100-606.
- [12]Monticello D J.Biodesulfurization and the upgrading of petroleum distillates[J].Curr Opin Biotechnol,2000,11:540-546.
- [13]GOU Zhong-xuan(缑仲轩),LIU Hui-zhou(刘会洲),LUO Ming-fang,et al(罗明芳等).Isolation and identification of specific desulfurization bacteria(专一性脱硫菌的分离与鉴定)[J].China Science(中国科学B辑),2002,32:397-405.
- [14]Morio K,Toshimitsu O,Yoshitaka I,et al.Desulfurization of alkylated forms of both dibenzothiophene and benzothiopheneby a single bacterial strain[J].FEMS Microbiology Letters,2000,187:123-126.