巫婷,景秋芳,叶波,沈永嘉

• 1:华东理工大学精细化工研究所结构可控先进功能材料及其制备教育部重点实验室
• 2:华东理工大学药学院
• 3:浙江三门腾龙化工有限公司

摘要(Abstract)：

利用2,4-二硝基氟苯为柱前衍生剂,建立了用高效液相色谱法测定普瑞巴林的方法。考察了光、反应温度、摩尔比和反应时间对衍生化反应的影响,确定了最佳衍生反应条件:在65℃水浴中避光反应75min。色谱柱为C18,流动相为50mmol·L-1KH2PO4(pH=3.0)/乙腈(30/70,v/v),检测波长为360nm,流速为1.0mL·min-1,柱温为35℃。实验结果:在8～48μg·mL-1范围内呈较好的线性关系(r=0.9998),回收率(100.03±1.10)%(n=9),不同浓度下日内精密度0.82%、0.77%和1.87%,8h内稳定性良好(RSD=1.92%),检测限低至0.8ng·mL-1。实验表明该方法简便、快速、准确、灵敏。通过对该方法的实际应用,三批样品测定值的RSD分别为0.59%、0.68%和1.04%,表明该方法能够用于测定普瑞巴林的含量。

关键词(KeyWords)： 普瑞巴林;2,4-二硝基氟苯;高效液相色谱;柱前衍生化

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 巫婷,景秋芳,叶波,沈永嘉

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