何可可,薛栋升,姚善泾

• 1:浙江大学化学工程与生物工程学系

摘要(Abstract)：

采用由实验室从海底淤泥中筛选得到曲霉Aspergillus sp.菌株,进行固体发酵生产胞外纤维二糖酶,粗酶液经过硫酸铵沉淀、超滤和两次离子交换层析纯化后,得到了电泳纯的纤维二糖酶,酶的比活力由10.98 U mg 1提高到131.21 U mg 1。考察了该酶的酶学性质,结果表明该酶的最适温度为65℃,最适pH为4.5,具有较好热稳定性和pH稳定性。盐浓度对酶活力有较大影响,当NaCl浓度为40 g L 1时具有最佳酶活,在20~150 g L 1的高NaCl浓度环境下的酶活均高于不含NaCl环境下的酶活,是典型的耐盐型酶。在高盐浓度下,该酶的热稳定性有较大的提高,半衰期比无盐条件下提高了2~3倍,说明纯化得到的纤维二糖酶具有明显的海洋酶耐盐和耐热特性,有更广的应用范围。

关键词(KeyWords)： 纤维二糖酶;分离纯化;耐盐酶;曲霉;热稳定性;pH稳定性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(21036005);; 浙江省科技厅公益技术研究社会发展项目(2011C33016)

作者(Author): 何可可,薛栋升,姚善泾

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