张辉,吴李花,邓超,陈怡,林春绵

• 1:浙江省计量科学研究院
• 2:浙江工业大学环境学院

摘要(Abstract)：

研究制备了L-半胱氨酸(L-cys)修饰的硒化镉(Cd Se)量子点,并以该量子点为荧光探针检测水中痕量Pb~(2+)。考察了量子点浓度(5.2×10~(-3)~5.2×10-5 mol×L~(-1))、反应时间(0~75 min)、pH(7.3~9)和干扰离子(Na~+、K~+、Ca~(2+)等)等因素对Pb~(2+)测定的影响。研究发现,当量子点的浓度为5.2×10-4 mol×L~(-1),pH值为8.0,反应时间为30 min时,检测效果最优,此条件下Pb~(2+)的检测线性区间为0.05~10 mg×L~(-1),检出限为0.02 mg×L~(-1)。

关键词(KeyWords)： 硒化镉;量子点;荧光探针;铅离子

Abstract:

Keywords:

基金项目(Foundation): 浙江省科学技术厅公益技术研究社会发展项目(2014C33053)

作者(Author): 张辉,吴李花,邓超,陈怡,林春绵

参考文献(References)：

• [1]JIANG He-mei(蒋和梅),YANG Dong-jing(杨东静),LI Nan(李楠),et al.Determination of lead(II)in water by FIA of Pb(II)-XO-CTMAB(Pb-XO-CTMAB流动注射法分析水样中的痕量铅)[J].Leather Science and Engineering(皮革科学与工程),2013,23(4):66-70.
• [2]CHEN Jian(陈坚),LIAO Jian-bo(廖建波),WEI Chao-hai(韦朝海).Analysis of chemical speciation and total amount of heavy metals in sediment by microwave digestion combined graphite furnace atomic absorption spectrometry(微波消解-石墨炉原子吸收法测定沉积物中重金属的全量及形态)[J].Chinese Journal of Analysis Laboratory(分析试验室),2014,33(7):803-807.
• [3]Liu Z Y,Huang S D.Determination of lead in sea-water with a graphite furnace atomic absorption spectrometer and an improved automatic on-line pre-concentration system[J].Spectrochimica Acta Part B,1995,50(2):197-203.
• [4]Chen Y,Li M T,Fu L L,et al.Simultaneous determination of trace cadmium and lead in single human hair by tungsten electrothermal vaporization-flame atomic fluorescence spectrometry[J].Microchemical Journal,2014,114:182-186.
• [5]SHI Min-shan(史岷山),FU Ji-hong(符继红),GAO Jin(高晶).Determination of Pb,As and Hg in lavandula angustifolia mill.of Xinjiang by atomic fluorescence spectrometry(原子荧光光谱法测定新疆薰衣草中的铅、砷和汞)[J].Journal of Analytical Science(分析科学学报),2014,30(2):247-50.
• [6]LIU Qing-ye(刘庆业),FAN Yan-yan(范燕燕),LING Shao-ming(凌绍明),et al.Spectrophotometric detection of lead(II)using aptamer modified nanogold particles(适体修饰金纳米粒子光度法检测铅(II))[J].Metallurgical Analysis(冶金分析),2010,4:58-61.
• [7]The Second Editorial Office of Standards Press of China(中国标准出版社第二编辑室).China standards compilation of environmental protection dealing with techniques to analyze the waste gas,the waste water,the waste residue:CJ/T69-1999.Municipal sewage-determination of lead-spectrophotometric method with dithizone[S](中国环境保护标准汇编废气废水废渣分析方法:CJ/T69-1999城市污水铅的测定双硫腙分光光度法[S]).Beijing Branch of Standards Press of China(北京中国标准出版社).2001:465-478.
• [8]Huang M R,Gu L G,Shi Y F,et al.Development of potentiometric lead ion sensors based on ionophores bearing oxygen/sulfurcontaining functional groups[J].Chinese Journal of Analytical Chemistry,2012,40(1):50-58.
• [9]Jaiswal J K,Mattoussi H,Mauro J M,et al.Long-term multiple color imaging of live cells using quantum dot bioconjugates[J].Nature Biotechnology,2003,21(l):47-51.
• [10]Sutherland A J.Quantum dots as luminescent probes in biological systems[J].Current Opinion in Solid State&Materials Science,2002,6(4):365-370.
• [11]Chan W C W,Nie S M.Quantum dots bioconjugates for ultrasensitive nonisotopic detection[J].Science,1998,5385(281):2016-2018.
• [12]Wang C,Qiang M A,Dou W C,et al.Synthesis of aqueous Cd Te quantum dots embedded silica nanoparticles and their applications as fluorescence probes[J].Talanta,2009,77(4):1358-1364.
• [13]Chen Y F,Rosenzweig Z.Luminescent Cd S quantum eots as selective ion probes[J].Analytical Chemistry,2002,74(19):5132-5138.
• [14]Cai C,Cheng H,Wang Y,et al.Mercaptosuccinic acid modified Cd Te quantum dots as a selective fluorescence sensor for Ag+determination in aqueous solutions[J].RSC Advances,2014,4(103):59157-59163.
• [15]ZHENG Ai-fang(郑爱芳),FANG Dian-jun(方典军),CHEN Jin-long(陈金龙).Preparation of functionalized Cd Se quantum dots and studies on the selective determination of mercury ion(功能化硒化镉量子点的制备以及作为汞离子荧光探针的研究)[J].Journal of Anqing Teachers College(Natural Science Edition)(安庆师范学院学报(自然科学版)),2008,14(2):39-44.
• [16]Bu X H,Zhou Y M,He M,et al.Bioinspired,direct synthesis of aqueous Cd Se quantum dots for high-sensitive copper(II)ion detection[J].Dalton Transactions,2013,42(43):15411-15420.
• [17]Ali E M,Zheng Y G,Yu H H,et a1.Uhrasensitive Pb2+detection by glutathione-capped quantum dots[J].Analytical Chemistry,2007,79(24):9452-9458.
• [18]YANG Fang-fang(杨芳芳),YU Jun-sheng(于俊生),XIE Ying(谢颖).Synthesis and characterization of water-soluble Cd Se/Cd S/Zn S core-shell quantum dots(水溶性的Cd Se/Cd S/Zn S量子点的合成及表征)[J].Chinese Journal of Inorganic Chemistry(无机化学学报),2008,24(7):1142-1147.
• [19]WANG Qiong(王琼),CHEN Jie-nan(陈介南),ZHANG Huai-yun(章怀云),et al.Optimization of preparation conditions and surface modification of water-soluble quantum dots(水溶性量子点制备条件的优化及表面修饰)[J].Materials Review(材料导报),2009,23(5):83-86.
• [20]LI Zhong(李众),ZHU Xin(祝欣),DONG Chao-qing(董朝青),et al.Aqueous synthetic approaches of luminescent quantum dots and their applications in chemical assays and bioassays(荧光量子点的水相合成及其在化学和生物分析中的应用)[J].Chemical Journal of Chinese Universities(高等学校化学学报),2010,30(10):1905-1915.
• [21]Dong M,Fu S,Liu S,et al.One-pot synthesis of Cd Se quantum dots in aqueous solution for biological labeling[J].Journal of the Chinese Chemical Society,2013,60(11):1328-1332.
• [22]LIU Hui(刘辉),LI Wen-you(李文友),YIN Hong-zong(尹洪宗),et al.Interaction between Cd S nanoparticles and cysteine(Cd S纳米粒子与半胱氨酸相互作用的研究)[J].Chemical Journal of Chinese Universities(高等学校化学学报),2005,26(9):1618-1622.
• [23]Susha A S,Javier A M,Parak W J,et al.Luminescent Cd Te nanocrystals as ion probes and p H sensors in aqueous solutions[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,2006,281(1-3):40-43.