应丹妮,陈焕芸,付岩,蔡海波,谭文松

• 1:华东理工大学生物反应器工程国家重点实验室

摘要(Abstract)：

为了优化免疫细胞体外培养体系实现其高效扩增,以自然杀伤细胞系(natural killer-92 cells,NK-92)为对象,通过氨基酸代谢分析及正交实验设计优化了关键代谢物浓度,并在有/无血清培养基中进行了验证。结果表明,谷氨酰胺丙酮酸钠浓度对NK-92细胞的体外扩增有显著影响,其最适浓度为13.0和2.5 mmol·L~(-1)。在此浓度下,有血清培养体系中NK-92细胞扩增15 d后,其扩增倍数可达到(3 712.85±225.74)倍,显著高于优化前对照组的(2 255.93±243.00)倍(p<0.05);其杀伤活性均值由57.23%提高到63.53%;无血清体系中NK-92细胞扩增15 d后,其扩增倍数可达到(3 193.59±199.99)倍,明显高于对照组的(1 917.16±242.87)倍(p<0.05),且显著提高了扩增后NK-92细胞中CD3~-CD56~+细胞比例和杀伤活性(p<0.05)。该结果可为免疫效应细胞无血清培养基的开发提供技术支持。

关键词(KeyWords)： 丙酮酸钠;谷氨酰胺;自然杀伤细胞;体外扩增

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 应丹妮,陈焕芸,付岩,蔡海波,谭文松

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