钱俊青,唐弋奥,李尚谦

• 1:浙江工业大学药学院

摘要(Abstract)：

为了获得适用于食品工业且固定化酶性能优异的固定化材料,规避在固定化材料制备过程中使用影响食品安全性的试剂,在无溶剂体系中以丙三醇改性四乙氧基硅烷(TEOS)获得改性硅烷凝胶。经过试验研究,获得的优化改性工艺条件为原料摩尔比四乙氧基硅烷(TEOS):丙三醇=1:0.25,固体酸催化剂3 g×(50 m L)~(-1) TEOS,反应温度50℃,反应时间72 h。优化工艺获得的改性四乙氧基硅烷亲水性明显增强,在水中的溶解量可达0.58 g×m L~(-1)。使用改性四乙氧基硅烷固定化假丝酵母脂肪酶,酶的催化活性得到良好保留,酶分子的结构刚性得到增强,和游离酶在相同条件下催化L-抗坏血酸棕榈酸酯的合成,改性硅烷凝胶固定化酶表现出比游离酶更好的催化活性。经改性后的硅烷凝胶是食品安全性良好的酶固定化材料。

关键词(KeyWords)： 丙三醇改性;硅烷凝胶;无溶剂体系;固定化;脂肪酶

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 钱俊青,唐弋奥,李尚谦

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