王园园,王康,金令凯,朱鸿强,施雪颖,陈敏

• 1:浙江工商大学 食品与生物工程学院，浙江 杭州 310018

摘要(Abstract)：

以绿色产色链霉菌为发酵菌株，采用单因素法在30 L发酵罐进行阿维拉霉素分批发酵的参数优化，并对优化后的发酵过程进行动力学研究。结果表明，在发酵转速为320 r·min^-1、通气量为2.0 vvm、发酵液初始pH为7.5的条件下，发酵开始后每间隔24 h流加0.1%的L-缬氨酸，罐内阿维拉霉素产量在发酵228 h可达到最高值1 571.04 mg ·L^-1。在此基础上，采用Logistic方程和Luedeking-Piret方程及基质消耗物料平衡方程分别研究菌体生物量、阿维拉霉素产量变化及底物总糖消耗与发酵时间的关系，建立阿维拉霉素发酵动力学模型，模型计算值与实验数据拟合度分别为0.989 6、0.990 9和0.981 9。该结果表明本研究所建立的菌体生长、产物合成和底物消耗模型能较好地反映阿维拉霉素分批发酵的动力学过程，可为其发酵过程的进一步优化及在线监测等提供理论依据。

关键词(KeyWords)： 绿色产色链霉菌;阿维拉霉素;发酵优化;动力学模型

Abstract:

Keywords:

基金项目(Foundation): 浙江省基础公益研究计划项目（LGN18C010001）。

作者(Author): 王园园,王康,金令凯,朱鸿强,施雪颖,陈敏

DOI: 10.3969/j.issn.1003-9015.2019.05.017

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