侯东杰,楼小玲,蔡礼年,陈治宏,张玮,陈冰冰,贠军贤

• 1:浙江工业大学机械工程学院
• 2:浙江工业大学绿色化学合成技术国家重点实验室培育基地

摘要(Abstract)：

针对有机酸生物合成过程中底物和产物抑制、细胞密度低和耐受性弱等问题，采用“结晶致孔-低温聚合”方法制备聚甲基丙烯酸羟乙酯-甲基丙烯酸丁酯半疏水晶胶微球，通过高速成像和数值模拟研究10 L搅拌式生物反应器(STR)内的悬浮行为，通过固载乳杆菌分析半疏水晶胶微球在乳酸和苯乳酸生物合成中的作用。结果表明，数值模拟得到晶胶微球的悬浮特性与高速成像实验结果基本一致，体积分数为3%的晶胶微球均匀悬浮所需最低搅拌转速为55 r·min~(-1)；在晶胶固载乳杆菌发酵联产乳酸和苯乳酸过程中，乳杆菌生物量达10.5 g·L~(-1)，相比于无载体发酵提高了84.2%；在第1次补料期间，乳酸的发酵周期大幅缩短至21 h，比无载体发酵周期缩短了50%。

关键词(KeyWords)： 半疏水晶胶微球;搅拌式生物反应器;悬浮行为;乳杆菌;发酵;数值模拟

Abstract:

Keywords:

基金项目(Foundation): 浙江省自然科学基金重大项目(LD21B060001);; 国家自然科学基金(22078296,21576240)

作者(Author): 侯东杰,楼小玲,蔡礼年,陈治宏,张玮,陈冰冰,贠军贤

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