许娜,关今韬,张颂红,贠军贤,关怡新,姚善泾

• 1:浙江工业大学化学工程学院绿色化学合成技术国家重点实验室培育基地
• 2:浙江大学化学工程与生物工程学院

摘要(Abstract)：

针对生物法合成苯乳酸存在成本高、高产菌株缺乏、工业化困难,稳定性低等问题,利用阴离子交换晶胶对副干酪乳杆菌的吸附作用,在发酵过程中形成复合生物催化剂,用于苯乳酸发酵和转化合成。对复合催化剂微观结构、形成过程特性、发酵和转化性能进行了测定。结果表明:发酵时投入晶胶可形成固载细胞的超大孔复合生物催化剂,不仅促进菌体生长,使生物量达7.10mg×m L~(-1),是同条件下无晶胶时的2.5倍,而且通过减轻底物抑制,将苯乳酸产量由0.71 mg×m L~(-1)提高到0.95 mg×m L~(-1);以浓度0.5、1和2 mg×m L~(-1)的苯丙氨酸为底物,采用复合催化剂转化生成苯乳酸的产量是相应全细胞催化剂时的3、2.5和1.5倍。

关键词(KeyWords)： 生物催化剂;苯乳酸;阴离子交换;晶胶

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(21576240,21036005);; 浙江省自然科学基金(LZ14B060001,LY16B060011)

作者(Author): 许娜,关今韬,张颂红,贠军贤,关怡新,姚善泾

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