汤星阳,夏颖,杨彬彬,夏黎明

• 1:浙江大学化学工程与生物工程学院

摘要(Abstract)：

土霉素作为兽用抗生素在禽、畜养殖业中应用广泛,严重危害环境和人体健康。采用漆酶对土霉素进行降解研究,通过介体筛选(丁香醛、香草醛、阿魏酸、对香豆酸、香草酮、ABTS、HBT),发现天然介体丁香醛和香草醛可有效促进漆酶对土霉素的降解,且效果要优于常用的人工合成介体ABTS和HBT。进一步研究表明:在相同介体浓度条件下(0.3 mmol·L~(-1)),复合介体的效果优于单一介体,其中由双天然介体丁香醛和香草醛配比而成的Syr/Van复合物对漆酶的作用显著。对漆酶-双天然介体系统降解土霉素的反应条件进行优化,在漆酶用量0.1 IU·mL~(-1)、50℃、120r·min~(-1)、pH 4.0的条件下反应4 h,漆酶-Syr/Van复合系统对土霉素的降解率高达95.14%。丁香醛、香草醛都是木质素降解过程的中间产物,采用此类廉价的天然介体替代人工合成介体ABTS、HBT,在经济和环保方面都具有明显的优势,该研究结果在环境治理方面具有良好的实际应用价值。

关键词(KeyWords)： 漆酶;天然介体;丁香醛;香草醛;土霉素;降解

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(21676247)

作者(Author): 汤星阳,夏颖,杨彬彬,夏黎明

参考文献(References)：

• [1]MA J,ZHU D,SHENG G D,et al.Soil oxytetracycline exposure alters the microbial community and enhances the abundance of antibiotic resistance genes in the gut of Enchytraeus crypticus[J].Science of the Total Environment,2019,673:357-366.
• [2]张憬,唐煜,梁斌.饲料中金霉素使用现状分析[J].饲料研究,2016,8:45-47.ZHANG J,TANG Y,LIANG B.Analysis of the current status of chlortetracycline use in feed[J].Feed Research,2016,8:45-47.
• [3]CHEN H,ZHOU Y,HU X,et al.Effects of chlortetracycline on biological nutrient removal from wastewater[J].Science of the Total Environment,2019,647:268-274.
• [4]SARMAH A K,MEYER M T,BOXALL A B.A global perspective on the use,sales,exposure pathways,occurrence,fate and effects of veterinary antibiotics(VAs)in the environment[J].Chemosphere,2006,65(5):725-759.
• [5]LI Y,FANG J,YUAN X,et al.Distribution characteristics and ecological risk assessment of tetracyclines pollution in the Weihe River,China[J].International Journal of Environmental Research and Public Health,2018,15(9):1803-1813.
• [6]JIANG Y,LI M,GUO C,et al.Distribution and ecological risk of antibiotics in a typical effluent-receiving river(Wangyang River)in north China[J].Chemosphere,2014,112:267-274.
• [7]WEI R,HE T,ZHANG S,et al.Occurrence of seventeen veterinary antibiotics and resistant bacterias in manure-fertilized vegetable farm soil in four provinces of China[J].Chemosphere,2019,215:234-240.
• [8]SOLOMON E I,SUNDARAM U M,MACHONKIN T E.Multicopper oxidases and oxygenases[J].Chemical Reviews,1996,96(7):2563-2606.
• [9]MATE D M,ALCALDE M.Laccase:a multi-purpose biocatalyst at the forefront of biotechnology[J].Microbial Biotechnology,2016,10(6):1457-1467.
• [10]TAHERAN M,NAGHDI M,BRAR S K,et al.Biodegradation of chlortetracycline by Trametes versicolor-produced laccase:By-product identification[J].Journal of Environmental Engineering,2018,144(4):04018018.
• [11]CANAS A I,CAMARERO S.Laccases and their natural mediators:biotechnological tools for sustainable eco-friendly processes[J].Biotechnology Advances,2010,28(6):694-705.
• [12]CALL H P,MUCKE I.History,overview and applications of mediated lignolytic systems,especially laccase-mediator-systems(Lignozym(R)-process)[J].Journal of Biotechnology,1997,53(2/3):163-202.
• [13]邱卫华,陈洪章.木质纤维素原料中漆酶天然介体的研究进展[J].生物工程学报.2014,30(5):726-733.QIU W H,CHEN H Z.Progress in natural laccase mediators from lignocelluloses[J].Chinese Journal of Biotechnology,2014,30(5):726-733.
• [14]BUGG T D,AHMAD M,HARDIMAN E M,et al.Pathways for degradation of lignin in bacteria and fungi[J].Natural Product Reports,2011,28(12):1883-1896.
• [15]ZENG S Q,ZHAO J,XIA L M.Simultaneous production of laccase and degradation of bisphenol A with Trametes versicolor cultivated on agricultural wastes[J].Bioprocess Biosystems Engineering,2017,40(8):1237-1245.
• [16]曾盛泉.Trametes versicolor产漆酶及其在有机污染物降解中的应用基础研究[D].杭州:浙江大学,2017.ZENG S Q.Basic research on laccase production with Trametes versicolor and its application in the degradation of organic pollutants[D].Hangzhou:Zhejiang University,2017.
• [17]郑茂佳,张恩栋,孙静茹,等.四环素类抗生素生物降解研究进展[J].天津农业科学,2018,24(6):72-76,85.ZHENG M J,ZHANG E D,SUN J R,et al.Biodegradation research progress of tetracyclines antibiotics[J].Tianjin Agricultural Sciences,2018,24(6):72-76,85.
• [18]WANG J,ZHOU B,GE R,et al.Degradation characterization and pathway analysis of chlortetracycline and oxytetracycline in a microbial fuel cell[J].RSC Advances,2018,8(50):28613-28624.