胡雪岩¹,宋克东¹,卢延国¹,李文芳¹,李丽颖¹,郑双双²,刘天庆¹

• 1:大连理工大学 精细化工国家重点实验室 干细胞与组织工程研究室，辽宁 大连 116024
• 2:郑州中科新兴产业技术研究院，河南 郑州 450000

摘要(Abstract)：

针对软骨特定结构缺损后不能自我修复的特点，通过调控单层原料质量比，构建具有复杂分层结构的壳聚糖/β-甘油磷酸钠/明胶（chitosan/β-sodium glycerophosphates/gelatin，Cs/GP/Gel）仿生复合梯度支架。通过对比物理性能优选适宜比例的支架材料，并将骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）接种到梯度支架上考察其生物相容性。结果表明，Cs/GP/Gel复合梯度支架具有良好的吸水性能[（584.24±3.79）%~（677.47±1.70）%]、孔隙率[（86.34±5.10）%~（95.20±2.86）%]和降解性能[（86.09±2.46）%~（92.48±3.86）%]。扫描电镜（SEM）结果表明，支架材料在纵向维度呈现出明显的生理分层结构和孔径梯度渐进性，可有效模拟真实软骨的天然生理分层结构。比例为9：1：5的Cs/GP/Gel复合梯度支架适于作为骨软骨的支撑材料。对BMSCs-支架复合物Live/Dead染色后发现，细胞在梯度支架材料上存活、分布及伸展良好。该仿生梯度支架为开发新型生物医用材料提供了重要依据。

关键词(KeyWords)： 仿生梯度支架;壳聚糖/β-甘油磷酸钠/明胶;骨髓间充质干细胞;骨/软骨组织工程

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金（31670978/31370991/21676041）；霍英东青年教师基金（132027）；辽宁省自然科学基金（20180510028）。

作者(Author): 胡雪岩¹,宋克东¹,卢延国¹,李文芳¹,李丽颖¹,郑双双²,刘天庆¹

DOI: 10.3969/j.issn.1003-9015.2019.05.014

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