刘晓贞,李云,梁云霄,张瑞丰

• 1:宁波大学材料科学与化学工程学院

摘要(Abstract)：

通过壳聚糖在块体Si O_2大孔材料孔壁上的吸附,制备二氧化硅/壳聚糖大孔复合材料(Si O_2/CS),经戊二醛交联后用于固定诺维信工业漆酶。优化漆酶固定化的实验条件并对比了游离漆酶和固定化漆酶的酶学性质。实验结果表明:在p H值为4.5、漆酶初始浓度为40 mg×m L~(-1)的条件下,固定化4 h效果最佳,固定化酶酶活回收率为85.5%;相对于游离漆酶,固定化漆酶的p H稳定性和热稳定性均得到明显提高,且具有良好的操作稳定性。应用固定化漆酶去除2,4-二氯苯酚(2,4-DCP),研究了降解时间、p H、温度和2,4-DCP初始浓度对其去除率的影响。在优化条件下,固定化漆酶对2,4-DCP(20 mg×L~(-1))的去除率为83.2%,固定化漆酶可重复使用,并且便于从反应体系中分离出来。

关键词(KeyWords)： SiO2大孔材料;壳聚糖;固定化;漆酶;2,4-二氯苯酚

Abstract:

Keywords:

基金项目(Foundation): 浙江省自然科学基金(LY12B01004);; 宁波市社会发展项目(2011C50052);; 宁波大学王宽城基金(XKL072)

作者(Author): 刘晓贞,李云,梁云霄,张瑞丰

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