高大海,梅乐和,盛清,许静,林东强,姚善泾

• 1:浙江大学化学工程与生物工程学系
• 2:浙江大学化学工程与生物工程学系
• 3:浙江理工大学生命科学学院
• 4:杭州中美华东制药有限公司

摘要(Abstract)：

利用生产纳豆激酶的Bacillus subtilis进行深层发酵。采用发酵液离心除菌,20%~60%饱和度的硫酸铵沉淀,Superdex75凝胶过滤层析和SPSepharose Fast Flow离子交换层析对活性组分进行分离提纯。用纤维平版法测定了活力,并用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳法(SDS-PAGE)对分离纯化的效果进行了检验。结果表明在SDS-PAGE中观察到单一条带,分子量27.7kD,最终纯化倍数和酶活回收率分别为8.4和49%。

关键词(KeyWords)： 纳豆激酶;分离纯化;凝胶层析;离子交换

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(20176050、30570411);; 浙江省科技项目(2004C33036);; 教育部回国人员基金项目;; 浙江省回国人员基金项目。

作者(Author): 高大海,梅乐和,盛清,许静,林东强,姚善泾

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