预处理方法对玉米秸秆发酵产氢的影响及其机理分析Effects of Pretreatments of Cornstalk on Hydrogen Production and Its Mechanisms Analysis
李领川,潘春梅,樊耀亭
摘要(Abstract):
研究了不同预处理方法对玉米秸秆发酵产氢气的影响和秸秆降解产氢的机理。实验分别采用酸解(AP)、酸解耦合固态酶解(AEP)、高温蒸煮(HP)和高温蒸煮耦合固态酶解(HEP)的玉米秸秆进行发酵产氢,分析预处理后秸秆累积产氢量与可溶性糖含量的关系。在此基础上,通过秸秆化学组成成分分析、傅里叶变换红外光谱(FT-IR)和X-射线衍射分析,探讨了秸秆降解的机理。结果表明,秸秆的累积产氢量与可溶性糖含量基本正相关,秸秆糖化效率是影响秸秆累积产氢量的主要因素。四种预处理方法主要作用于秸秆半纤维素和纤维素的无定型区,预处理过程皆在不同程度上提高了秸秆的结晶度,并在极大程度上提高了玉米秸秆的累积产氢量。其中AEP方法预处理秸秆效果最好,累积产氢量达到了226.1 m L·(g·TS)-1。
关键词(KeyWords): 玉米秸秆;预处理;发酵产氢;机理
基金项目(Foundation): 国家自然科学基金河南省人才培养联合基金(U1204522);; 河南省高校科技创新人才支持计划项目(13HASTIT033)
作者(Author): 李领川,潘春梅,樊耀亭
参考文献(References):
- [1]Penteado E D,Lazaro C Z,Sakamoto I K,et al.Influence of seed sludge and pretreatment method on hydrogen production in packed-bed anaerobic reactors[J].International Journal of Hydrogen Energy,2013,38(14):6137-6145.
- [2]Kim D H,Kim S H,Jung K W,et al.Effect of initial p H independent of operational p H on hydrogen fermentation of food waste[J].Bioresource Technology,2011,102(18):8646-8652.
- [3]Patel S K S,Kumar P,Kalia V C.Enhancing biological hydrogen production through complementary microbial metabolisms[J].International Journal of Hydrogen Energy,2012,37(14):10590-10603.
- [4]Vasconcelos L R S,Cammarota M C,Oliveira T C,et al.Pentoses,hexoses and glycerin as substrates for biohydrogen production:an approach for Brazilian biofuel integration[J].International Journal of Hydrogen Energy,2013,38(7):2986-2997.
- [5]Gadhe A,Sonawane S S,Varma M N.Kinetic analysis of biohydrogen production from complex dairy wastewater under optimized condition[J].International Journal of Hydrogen Energy,2014,39(3):1306-1314.
- [6]Han W,Chen H,Jiao AY,et al.Biological fermentation hydrogen and ethanol production using continuous stirred tank reactor[J].International Journal of Hydrogen Energy,2012,37(1):843-847.
- [7]Arreolavargas J,Celis LB,Buitrón G,et al.Hydrogen production from acid and enzymatic oat straw hydrolysates in an anaerobic sequencing batch reactor:performance and microbial population analysis[J].International Journal of Hydrogen Energy,2013,38(32):13884-13894.
- [8]Pan C M,Zhang S F,Fan Y T,et al.Bioconversion of corncob to hydrogen using anaerobic mixed microflora[J].International Journal of Hydrogen Energy,2010,35(17):2663-2669.
- [9]Clark I C,Zhang R H,Upadhyaya S K.The effect of low pressure and mixing on biological hydrogen production via anaerobic fermentation[J].International Journal of Hydrogen Energy,2012;37(15):11503-11513.
- [10]Assawamongkholsiri T,Reungsang A,Pattra S.Effect of acid,heat and combined acid-heat pretreatments of anaerobic sludge on hydrogen production by anaerobic mixed cultures[J].International Journal of Hydrogen Energy,2013,38(14):6146-6153.
- [11]ZHANG Shu-fang(张淑芳),PAN Chun-mei(潘春梅),FAN Yao-ting(樊耀亭),et al.Bioconversion of corncob to hydrogen using anaerobic fermentation(玉米芯发酵法生物制氢)[J].Chinese Journal of Biotechnology(生物工程学报),2008,24(6):1085-1090.
- [12]XING Yan(杏艳),MA Hong-cui(马红翠),FAN Yao-ting(樊耀亭),et al.Cellulose-hydrogen production from corn stalk biomass by anaerobic fermentation(秸秆类生物质发酵法生物产氢的研究)[J].Chinese Science Bulletin(科学通报),2009,54(5):648-654.
- [13]Li D M,Chen H Z.Biological hydrogen production from steam-exploded straw by simultaneous saccharification and fermentation[J].International Jouranl of Hydrogen Energy,2007,32(12):1742-1748.
- [14]Zhang M L,Fan Y T,Xing Y,et al.Enhanced biohydrogen production from cornstalk wastes with acidification pretreatment by mixed anaerobic cultures[J].Biomass and Bioenergy,2007,31(4):250-254.
- [15]Xing Y,Fan S Q,Zhang J N,et al.Enhanced bio-hydrogen production from corn stalk by anaerobic fermentation using response surface methodology[J].International Journal of Hydrogen Energy,2011,36(20):12770-12779.
- [16]Pan C M,Ma H C,Fan Y T,et al.Bioaugmented cellulosic hydrogen production from cornstalk by integrating dilute acid-enzyme hydrolysis and dark fermentation[J].International Journal of Hydrogen Energy,2011,36(8):4852-4862.
- [17]Avci A,Saha B C,Dien B S,et al.Response surface optimization of corn stover pretreatment using dilute phosphoric acid for enzymatic hydrolysis and ethanol production[J].Bioresource Technology,2013,130:603-612.
- [18]Wang Z J,Zhu J Y,Ronald S Z J,et al.Ethanol production from poplar wood through enzymatic saccharification and fermentation by dilute acid and SPORL pretreatments[J].Fuel,2012,95:606-614.
- [19]Ayse A,Badal C A,Gregory J K,et al.Dilute sulfuric acid pretreatment of corn stover for enzymatic hydrolysis and efficient ethanol production by recombinant Escherichia coli FBR5 without detoxification[J].Bioresource Technology,2013,142:312-319..
- [20]Fan Y T,Li C L,Lay J J,et al.Optimization of initial substrate and p H levels for germination of sporing hydrogen-producing anaerobes in cow dung compost[J].Bioresource Technology,2004;91(2),189-193.
- [21]Fan Y T,Zhang Y H,Zhang S F,et al.Efficient conversion of wheat straw wastes into biohydrogen gas by cow dung compost[J].Bioresource Technology,2006,97(3):500-505.
- [22]Li Q,Liu C Z.Co-culture of Clostridium thermocellum and Clostridium thermosaccharolyticum for enhancing hydrogen production via thermophilic fermentation of cornstalk waste[J].International Journal of Hydrogen Energy,2012,37(14):10648-10654.
- [23]Han H L,Wei L L,Liu B Q,et al.Optimization of biohydrogen production from soybean straw using anaerobic mixed bacteria[J].International Journal of Hydrogen Energy,2012,37(17):13200-13208.
- [24]BAI Zhen-zhen(白真真),WU Li-yang(吴黎阳),FAN Yao-ting(樊耀亭),et al.Effect of solid fermentation on performance of hydrogen production(固态发酵对秸秆废弃物产氢性能的影响)[J].J Chem Ind and Eng(China)(化工学报),2011,62(10):2439-2444.