周苏果,李大方,沈忱,熊春华

• 1:浙江工商大学应用化学系

摘要(Abstract)：

比较了8种大孔吸附树脂D101、AB-8、NKA-9、D4020、S-8、200702、H103、NKA-Ⅱ对菊米总黄酮的吸附性能,以大孔吸附树脂对菊米总黄酮的吸附率、洗脱率为评价指标,筛选出合适的大孔吸附树脂分离纯化菊米总黄酮,并以静态实验、动态试验考察大孔树脂对菊米总黄酮的分离纯化效果及影响因素,优化吸附和解吸条件。结果表明:200702中极性树脂分离纯化菊米总黄酮效果较好,其最佳吸附工艺为:上样液pH 5~6,质量浓度0.35 mg?mL-1,上样液流速3.0 mL?min-1,最佳洗脱工艺为:70%乙醇溶液30 mL,洗脱速率2.5 mL?min-1,通过本工艺菊米总黄酮纯度达83.5%。

关键词(KeyWords)： 菊米;大孔树脂;黄酮;纯化

Abstract:

Keywords:

基金项目(Foundation): 浙江省科技厅计划项目(2006C31029);; 浙江省教育厅科研项目(Y201223624)

作者(Author): 周苏果,李大方,沈忱,熊春华

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