武强,林东强,翁叶靖,姚善泾

• 1:化学工程联合国家重点实验室(浙江大学)
• 2:浙江大学化学工程与生物工程学系

摘要(Abstract)：

以2-巯基-1-甲基咪唑(MMI)为混合模式配基,人血免疫球蛋白G(IgG)为模型蛋白,探讨混合模式配基双水相系统分离抗体的新方法,主要包括MMI-聚乙二醇(PEG)的制备和IgG分配研究。首先优化了PEG4000的环氧氯丙烷活化反应条件,环氧氯丙烷和PEG比例为3.5:1,三氟化硼乙醚溶液加量为0.05 mL g 1PEG,开环反应时间为8 h;40%氢氧化钠溶液加量为0.15 mL g-1PEG,闭环反应时间为4 h,环氧基团接入量可达到460μmol g-1PEG,偶联配基MMI,得到MMI-PEG混合模式配基。进一步考察了IgG在PEG4000/(NH4)2SO4双水相系统中的分配,发现添加MMI-PEG配基有效提高了IgG分配系数,加入适量NaCl,分配系数达到了17.1。结果表明,混合模式配基双水相萃取可以实现IgG的上相富集,为抗体分离提供了一个新途径。

关键词(KeyWords)： 双水相系统;混合模式配基;2-巯基-1-甲基咪唑;免疫球蛋白G;分配系数

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(21276228,21036005);; 浙江省自然科学基金(LR12B06003)

作者(Author): 武强,林东强,翁叶靖,姚善泾

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