王良华,陈芳,贠军贤,姚克俭,姚善泾

• 1:浙江大学化学工程与生物工程学系
• 2:浙江工业大学化学工程与材料学院
• 3:绿色化学合成国家重点实验室培育基地

摘要(Abstract)：

采用原位接枝法,将3-烯丙氧基-2-羟基-1-丙磺酸钠(AHPSA)接枝到聚丙烯酰胺基晶胶基质孔隙内表面,制备得到了阳离子交换型连续床用的带磺酸基超大孔晶胶介质。通过脉冲示踪法测量停留时间分布(RTD),得到了晶胶介质的理论等板高度(HETP)。通过测量一定压差下流经晶胶床柱的液量,得到晶胶介质的渗透率。用溶菌酶作为模型蛋白测量晶胶介质的吸附容量。考察了单体浓度、接枝反应时间等反应条件对晶胶介质的HETP、渗透率、蛋白质吸附容量等性能的影响。结果表明:接枝AHPSA的阳离子交换晶胶介质的HETP基本不受接枝反应时间和单体浓度的影响,其渗透率随接枝反应时间增大略有减小,其对溶菌酶的吸附容量与接枝单体的浓度成正比,受接枝反应时间的影响较小。

关键词(KeyWords)： 生物分离;超大孔连续床;原位接枝;晶胶;阳离子交换

Abstract:

Keywords:

基金项目(Foundation): 浙江省自然科学基金资助项目(Y405054)

作者(Author): 王良华,陈芳,贠军贤,姚克俭,姚善泾

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