当归中阿魏酸的提取和纯化研究Study on the Extraction and Purification of Ferulic Acid in Angelica Sinensis
刘芝兰,王娟,沈平,沈永嘉
摘要(Abstract)：
以当归中主要活性物质阿魏酸的含量为指标,采用单因素及均匀设计实验的方法,确定了微波辅助提取当归中阿魏酸的最优工艺条件:乙醇浓度90%,微波功率850W,液固比6:1(mL:g),辐射时间9min,粒径大小为2mm,浸泡时间30min。结果表明:干浸膏中阿魏酸含量为0.212%,转移率为70.19%,均优于传统加热提取和超声提取方法,而且所用的时间分别是传统加热提取方法和超声提取方法的3/40和3/10。将高速逆流色谱技术应用于上述干浸膏中阿魏酸的分离纯化,经过一次分离得到的阿魏酸纯度为98.2%(HPLC)。HSCCC的溶剂系统组成为:正己烷-乙酸乙酯-甲醇-水(3:7:5:5)。用核磁共振和质谱表征了所得阿魏酸的化学结构。
关键词(KeyWords)： 微波辅助提取;阿魏酸;当归;均匀设计;高速逆流色谱
基金项目(Foundation):
作者(Author): 刘芝兰,王娟,沈平,沈永嘉
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