超临界CO2下AOT/CTAB混合反胶团体系萃取乳酸钙中蛋白质的研究Investigation of Extracting Proteins in Calcium Lactate by AOT/CTAB Mixed Reverse Microemulsions in Supercritical Carbon Dioxide
肖恩荣,李定或,丁一刚,吴元欣
摘要(Abstract)：
研究了超临界CO2下AOT/CTAB混合反胶团体系萃取乳酸钙中影响产品品质的杂蛋白质。并且分析了萃取压力P、温度T、AOT用量VAOT及AOT/CTAB摩尔混合比nAOT/nCTAB、水量VH2O、pH值等各因素对于该体系中蛋白质的萃取率的影响。通过正交试验得出最佳操作条件:P=25MPa,T=45℃,VAOT=0.15mL.(g乳酸钙)-1,nAOT/nCTAB=300:1,VH2O=1.6mL水.(g乳酸钙)-1,pH=4。在此操作条件下,蛋白质的去除率可达70%。
关键词(KeyWords)： 超临界CO_2;反胶团;乳酸钙;蛋白质;二(2-乙基已基)丁二酸酯磺酸钠(AOT);十六烷基三甲基溴化铵(CTAB)
基金项目(Foundation): 湖北省科技攻关项目(202AA108B01)。
作者(Author): 肖恩荣,李定或,丁一刚,吴元欣
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