梁猛,陈浩,魏坤

• 1:华南理工大学材料科学与工程学院
• 2:温州生物材料与工程研究所

摘要(Abstract)：

通过乳液溶剂挥发法制备复合微球,然后对微球进行烧结处理得到微球支架。通过调节β-tricalcium phosphate(β-TCP,β型磷酸三钙)的添加量来调节微球支架的抗压强度、孔隙率和孔隙连通率,进而得到一种在抗压强度上满足人体骨组织修复的需求且孔隙率和孔隙连通率均利于细胞进入支架内部的微球支架。经过测试观察,在poly(lactic-co-glycolic acid)(PLGA,聚乳酸-羟基乙酸共聚物)微球中分别加入0.1、0.2、0.3和0.4 gβ-TCP后,与纯PLGA微球相比发现添加有β-TCP的微球表面粗糙,且粗糙度随着β-TCP量的增加而增大。在70℃下经过1 h烧结后,发现随着β-TCP量的增多,支架中微球之间的粘结程度逐渐下降,孔隙率和平均孔径逐渐增加。当β-TCP为0.3 g时,平均孔径达到84.34μm,超过细胞进入支架的最小孔径80μm,利于细胞在支架内部生长。随着β-TCP增多,支架的抗压强度先增大后减小,当β-TCP为0.2 g时,抗压强度最大,为(6.05±0.74)MPa。这是因为β-TCP的抗压强度较高,复合到PLGA微球当中可以提升支架的抗压强度。当β-TCP含量过高时,微球之间粘结程度过小,连接不牢固,进而降低了支架的抗压强度。通过检测得出支架的细胞相容性会随着β-TCP的增多而增强。从抗压强度、支架平均孔径、孔隙率和细胞相容性等方面综合分析,PLGA/β-TCP 0.3微球支架在骨组织修复方面具备较好的应用价值,可望成为一种理想的骨修复材料。

关键词(KeyWords)： 聚乳酸-羟基乙酸共聚物;β-型磷酸三钙;孔隙率;抗压强度;细胞相容性

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 梁猛,陈浩,魏坤

参考文献(References)：

• [1]MENG Yong-chun(孟永春).Research on the interaction mechanical between the collagen and bioactive glass and their composite as bone repair material(胶原与生物活性玻璃作用机理及其复合骨修复材料的研究)[D].Guangzhou(广州):South China University of Technology(华南理工大学),2010.
• [2]Masson N C,De Souza E F,Galembeck F.Calcium and iron(III)polyphosphate gel formation and aging[J].Colloid Surface A:Physicochemical and Engineering Aspects,1997,121(2-3):247-255.
• [3]Roy T D,Simon J L,Ricci J L,et al.Performance of degradable composite bone repair products made via three-dimensional fabrication techniques[J].Journal of Biomedical Materials Research Part A,2003,66A(2):283-291.
• [4]Specchia N,Pagnotta A,Greco F.Can porosity influence the osteoconductive properties of synthetic hydroxyapatite?[C].Switzerlard,Key Engineering Materials,2000:355-358.
• [5]Wang M.Developing bioactive composite materials for tissue replacement[J].Biomaterials,2003,24(13):2133-2151.
• [6]Zhou X,Yan Y N,Wang S G,et al.Fabrication of porous scaffolds for bone tissue engineering via low-temperature deposition[J].Scripta Materialia,2002,46(11):771-776.
• [7]Farooq A,Yar M,Khan A S,et al.Synthesis of piroxicam loaded novel electrospun biodegradable nanocomposite scaffolds for periodontal regeneration[J].Materials Science and Engineering:C Materials for Biological Application,2015,56(1):104-113.
• [8]Zhang J,Deng A P,Yang Y,et al.HPLC detection of loss rate and cell migration of HUVECs in a proanthocyanidin cross-linked recombinant human collagen-peptide(RHC)-chitosan scaffold[J].Materials Science and Engineering:C Materials for Biological Application,2015,56:555-563.
• [9]SONG Ke-dong(宋克东),LIU Tian-qing(刘天庆),GUO Wen-hua(郭文华),et al.Sustained releasable chitosan scaffolds embedded with PLGA microspheres(包埋PLGA微球的可控释壳聚糖支架材料的研究)[J].Journal of Chemical Engineering of Chinese Universities(高校化学工程学报),2010,24(6):985-992.
• [10]Ma Z G,Zhao N,Xiong C D.Degradation and miscibility of poly(DL-lactic acid)/poly(glycolic acid)composite films:effect of poly(DL-lactic-co-glycolic acid)[J].Bulletin of Materials Science,2012,35(4):575-578.
• [11]Moreira A D,Sader M S,Soares G D,et al.Strontium incorporation on microspheres of alginate/β-tricalcium phosphate as delivery matrices[J].Materials Research Ibero American Journal of Materials,2014,17(4):967-973.
• [12]Lee H B,Lim H J,Cho M,et al.Clinical significance of beta-tricalcium phosphate and polyphosphate for mastoid cavity obliteration during middle ear surgery:Human and Animal Study[J].Clinical and Experimental Otorhinolaryngology,2013,6(3):127-134.
• [13]Rodrigues P L,Almeida F S,Motisuke M,et al.Effect of alumina addition on the physical and mechanical properties ofβ-tricalcium phosphate[J].Ceramic,2012,58(347):368-373.
• [14]Yang L,Sun H Y,Qi N M,et al.The effects of beta-tricalcium phosphate 3D scaffold in-situ cryopreservation on the migration rate and osteogenic ability of mesenchymal stem cells[J].Biotechnology and Bioprocess Engineering,2012,17(1):195-202.