肖安风,杨尚彤,倪辉,杨哲,蔡慧农

• 1:集美大学生物工程学院
• 2:福建省食品微生物与酶工程重点实验室
• 3:厦门市食品生物工程技术研究中心

摘要(Abstract)：

根据肌苷合成的代谢调控机理,选择五种对肌苷合成有促进作用的代谢调控剂—氟化钠、次黄嘌呤、葡萄糖酸钙、氯化钙和柠檬酸钠,并确定了其最适添加量。进而组合添加这些代谢调控剂,对肌苷生物合成过程的多个关键代谢调控节点进行调控,增大流向肌苷合成的代谢流,使肌苷产量得到提高。研究发现,在培养基中组合添加这些代谢调控剂,所得肌苷产量比单一添加代谢调控剂时的肌苷产量有显著提升。其中当组合添加2 mg·L-1 NaF、0.15%次黄嘌呤、15 g·L-1葡萄糖酸钙及10 mg·L-1 CaCl2时,肌苷产量达到了14.01 g·L-1,相比对照组的肌苷产量提高了3.39倍,协同效应显著。继而,对肌苷合成的代谢网络各分支途径的代谢通量进行了测定,证实了组合添加代谢调控剂有利于葡萄糖代谢流由EMP途径向HMP途径发生迁移,实现对葡萄糖主体代谢流分布的调整,促使肌苷合成代谢流增加,从而有利于肌苷产量的进一步提升。

关键词(KeyWords)： 枯草芽孢杆菌;肌苷;发酵过程;代谢调控剂;代谢通量分析

Abstract:

Keywords:

基金项目(Foundation): 福建省产学研重大专项项目(2010N5009);; 福建省自然科学基金项目(2011J01224);; 集美大学中青年创新团队专项基金项目(2010A006)资助

作者(Author): 肖安风,杨尚彤,倪辉,杨哲,蔡慧农

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