漆酶在介孔分子筛MCM-41上的固定化研究Study of Immobilization of Laccase on Mesoporous Molecular Sieve MCM-41
王炎,郑旭翰,赵敏
摘要(Abstract)：
以介孔分子筛MCM-41作为漆酶固定化的载体,采用物理吸附法进行固定化,考察了时间、pH和给酶量对固定化效果的影响,并对固定化酶的活性及其稳定性进行了研究,讨论了影响固定化过程和固定化酶性质的主要原因。结果显示,在pH为3时,酶和载体比例为62.5mg·g·1时吸附12h固定化效果最好,固定化酶活性回收率为50%;与游离漆酶相比,MCM-41固定化漆酶的最适反应pH略有升高,最适温度没有变化,其pH稳定性和热稳定性都显著优于游离漆酶,固定化漆酶具有可重复操作的性质,与底物反应反复操作10批次后剩余活性为40%。以上表明,MCM-41作为固定化漆酶的一种新型载体材料,有利于改善漆酶的稳定性和实用性。
关键词(KeyWords)： 介孔分子筛;MCM-41;漆酶;固定化
基金项目(Foundation): 国家自然科学基金资助项目(30170775,30671702)
作者(Author): 王炎,郑旭翰,赵敏
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