张军伟,陈小萍,芮昌春,常宽

• 1:江南大学化学与材料工程学院

摘要(Abstract)：

为了探讨配位体交换树脂在分离低聚木糖(XOS)中木二糖(X2)的性能及作用机制，研究了不同金属离子对树脂分离效能的影响规律及其与糖分子的结合能，探讨了各组分在树脂上的吸附行为。研究发现，Na~+因具有适中的电子云分布和优良的空间匹配能力，使其负载型树脂表现出良好的分离效果，木三糖/木二糖(X3/X2)与木二糖/葡萄糖(X2/Glu)的分离度分别达到0.47和0.81，优于其他离子型树脂。热力学分析表明，X2的吸附过程为吸热熵驱动，竞争型Modified-Langmuir模型能较好地描述其等温吸附行为。分离机制主要源于金属离子与糖羟基之间的特异性配位作用，并协同树脂的孔径筛分效应及组分间的竞争性吸附过程，实现较好选择性分离。研究结论可为XOS中特定寡糖组分的高效纯化提供理论参考。

关键词(KeyWords)： 低聚木糖;木二糖;配位体交换树脂;分离性能;作用机制

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助(21307042);; 江苏省博士后基金(1601073B)

作者(Author): 张军伟,陈小萍,芮昌春,常宽

参考文献(References)：

• [1]张庆明，徐云燕，贾佳，等.高品质低聚木糖对小鼠肠道菌群及免疫功能影响的研究[J].药学与临床研究，2023,31(4):321-325.ZHANG Q M,XU Y Y,JIA J,et al. Study on the effect of high-quality xylo-oligosaccharides on intestinal flora and immune function of mice[J]. Pharmaceutical and Clinical Research,2023,31(4):321-325.
• [2]RINI D M,NAKAMICHI Y,MORITA T,et al. Xylobiose treatment strengthens intestinal barrier function by regulating claudin 2 and heat shock protein 27 expression in human Caco-2 cells[J]. Journal of the Science of Food and Agriculture, 2024, 104(4):2518-2525.
• [3]陈佩瑶，周辉，王锋，等.沅江芦笋低聚木糖制备工艺优化、结构鉴定及抗氧化性研究[J].食品科技，2024,49(4):201-209,228.CHEN P Y,ZHOU H,WANG F,et al. Optimization of preparation process,structural identification and antioxidant activity of xyloOligosaccharides from yuanjiang asparagus[J]. Food Science and Technology,2024,49(4):201-209,228.
• [4]CHEN Y X, XIE Y N, AJUWON K M, et al. Xylo-oligosaccharides, preparation and application to human and animal health:A review[J]. Frontiers in Nutrition,2021,8:731930.
• [5]PLUTA R,U??AMEK-KOZIO??M,JANUSZEWSKI S,et al. Gut microbiota and pro/prebiotics in Alzheimer′s disease[J].Aging-US,2020,12(6):5539-5550.
• [6]LIM S,KIM E,SHIN J H,et al. Xylobiose prevents high-fat diet induced mice obesity by suppressing mesenteric fat deposition and metabolic dysregulation[J]. Molecules,2018,23(3):705.
• [7]赵剑，解静聪，姜越，等.重组木聚糖酶的高密度发酵及其定向制备低聚木糖[J].林产化学与工业，2024,44(1):97-104.ZHAO J, XIE J C, JIANG Y, et al. High-density fermentation of recombinant xylanase and its directed preparation of xylooligosaccharides[J]. Chemistry and Industry of Forest Products,2024,44(1):97-104.
• [8]胡蝶，徐道铸，鲁志毅，等.木聚糖酶Xyn11A与阿拉伯呋喃糖苷酶Abf62A协同水解麦秆木聚糖生物合成低聚木糖[J].合成生物学，2025,6(4):972-986.HU D,XU D Z,LU Z Y,et al. Synergistic hydrolysis of wheat straw Xylan by xylanase xyn11A and arabinofuranosidase Abf62A for biosynthesis of xylo-oligosaccharides[J]. Synthetic Biology Journal,2025,6(4):972-986.
• [9]吕惠生，冯永鑫，周锦怡，等.苦荞活性组分超临界流体工业色谱提纯工艺[J].高校化学工程学报，2020,34(3):626-632.LYU H S, FENG Y X, ZHOU J Y, et al. Purification process of active components from Tartary buckwheat by supercritical fluid industrial chromatography[J]. Journal of Chemical Engineering of Chinese Universities,2020,34(3):626-632.
• [10]TAIPABU M I,HSIEH Y L,VISWANATHAN K,et al. Organic ternary mixture separation and purification using chromatography:Design,operation,and optimization[J]. Journal of the Taiwan Institute of Chemical Engineers,2023,153:105182.
• [11]王炳英，丁玉珍，刘钢，等.特医食品中植物源新食品原料测定方法研究进展[J].食品安全质量检测学报，2021,12(2):740-745.WANG B Y,DING Y Z,LIU G,et al. Research progress on determination methods of plant-derived novel food ingredients in medical foods[J]. Journal of Food Safety and Quality,2021,12(2):740-745.
• [12]PREECHAKUN T, PONGCHAIPHOL S, CHAMPREDA V, et al. Separation of xylose and xylooligosaccharides from sugarcane trash extracted from hydrothermal process and followed by ion-exchange resin technique[J]. Biomass and Bioenergy, 2025, 197:107826.
• [13]SVOBODA T, HENKE S, GILLAROV??S, et al. Model-based scaling of preparative chromatographic separation of sucrose and glucose using bilevel parameter estimation for equilibrium dispersive model with nonlinear isotherm[J]. Journal of Chromatography A,2025:466135.
• [14]张丹丹，矫芮文，米春孝，等.用于分离单糖与寡糖的亲水色谱材料制备及应用进展[J].食品科学，2024,45(9):261-271.ZHANG D D,JIAO R W,MI C X,et al. Preparation and application progress of hydrophilic chromatographic materials for separation of monosaccharides and oligosaccharides[J]. Food Science,2024,45(9):261-271.
• [15]陈志，李倩倩，孙加燕，等.色谱图中拖尾峰峰宽及分离度的新计算方法及其应用[J].中国医药工业杂志，2023,54(9):1374-1381.CHEN Z,LI Q Q,SUN J Y,et al. A new calculation method and its application for the peak width and resolution of tailed peaks in chromatograms[J]. Chinese Journal of Pharmaceuticals,2023,54(9):1374-1381.
• [16]张军伟，刘月朗，孙震，等.配位体交换树脂分离乙醇酸中无机盐的性能及过程模拟[J].高校化学工程学报，2023,37(6):917-924.ZHANG J W,LIU Y L,SUN Z,et al. Performance and process simulation of ligand exchange resin for separating inorganic salts from glycolic acid[J]. Journal of Chemical Engineering of Chinese Universities,2023,37(6):917-924.
• [17]沈醉，林东强，姚善泾.逆体积排阻层析法测定层析介质的孔径分布[J].化工学报，2010,61(4):867-874.SHEN Z, LIN D Q, YAO S J. Determination of pore size distribution of chromatographic media by inverse size exclusion chromatography[J]. CIESC Journal,2010,61(4):867-874.
• [18]NIEZEN L E, KRUIJSWIJK J D, VAN HENTEN G B, et al. Principles and potential of solvent gradient size-exclusion chromatography for polymer analysis[J]. Analytica Chimica Acta,2023,1253:341041.
• [19]XU X, CHEN Y, WANG Z, et al. Nanofiltration membranes with ultra-high negative charge density for enhanced anion sievingand removal of organic micropollutants[J]. Nature Water,2025:1-10.
• [20]沈晓岩，陈亮，齐慧，等.水热法制备低聚木糖及其分离纯化研究进展[J].食品与机械，2025,41(6):228-235.SHEN X Y, CHEN L, QI H, et al. Research progress on preparation of xylooligosaccharides by hydrothermal method and their separation and purification[J]. Food&Machinery,2025,41(6):228-235.
• [21]KIM D,YU J H,HONG K S,et al. Green production of low-molecular-weight xylooligosaccharides from oil palm empty fruit bunch via integrated enzymatic polymerization and membrane separation for purification[J]. Separation and Purification Technology,2022,293:121084.
• [22]肖江，王佳凤，黄致远，等.高效液相色谱法检测海藻糖中有关物质[J].食品与药品，2025,27(4):380-384.XIAO J, WANG J F, HUANG Z Y, et al. Determination of related substances in trehalose by high performance liquid chromatography[J]. Food and Drug,2025,27(4):380-384.
• [23]ZHANG X, LIU L, JIANG B, et al. Efficient adsorption of alginate oligosaccharides by ion exchange resin based on molecular simulation and experiments[J]. Separation and Purification Technology,2023,317:123942.
• [24]王朝旭，王端达，王树涛，等.有序多孔材料在色谱分离分析领域的应用[J].色谱，2025,43(6):594-605.WANG C X,WANG D D,WANG S T,et al. Application of ordered porous materials in the field of chromatographic separation and analysis[J]. Chinese Journal of Chromatography,2025,43(6):594-605.
• [25]WU H, WANG D, SCHWARTZ D K. Connecting hindered transport in porous media across length scales:From single-pore to macroscopic[J]. The Journal of Physical Letter,2020,11(20):8825-8831.
• [26]JIAO L, WEI W, LIAO C Y, et al. Quaternary ammonium-functionalized rosin-derived resin for the high-performance capture of caramels:Experiments and quantum chemical theory simulations[J]. Jornal of Hazardous Materials,2024,461:132633.
• [27]MEYER V R. Ion-exchange Chromatography[M]. St. Gallen:John Wiley and Sons Ltd.,2010.
• [28]CRUZ-TIRADO J P, CABANILLAS A, SICHE R, et al. Bleaching of sugar cane juice using a food-grade adsorber resin and explained by a kinetic model describing the variation in time of the content of adsorbate[J]. Food Science and Technology International,2018,24(3):264-274.
• [29]ASSAF Z,WURSTER D E. Molecular dynamics of hydration shells of adsorbates in entropy-driven adsorption(hydrophobic bonding)to activated carbon surfaces[J]. Journal of Pharmaceutical Sciences,2024,113(4):982-989.
• [30]吴坚，赵长多，陈嘉宇，等.钒，钨离子在D201树脂上的吸附分离性能[J].高校化学工程学报，2020,34(4):897-903.WU J, ZHAO C D, CHEN J Y, et al. Adsorption and separation performance of vanadium and tungsten Ions on D201 resin[J].Journal of Chemical Engineering of Chinese Universities,2020,34(4):897-903.
• [31]SONG M K,JIAO P F,QIN T T,et al. Recovery of lactic acid from the pretreated fermentation broth based on a novel hyper-crosslinked meso-micropore resin:Modeling[J]. Bioresource Technology,2017,241:593-602.
• [32]KOU J W, LI L, XIANG H L, et al. Competitive and cooperative, of glucose and sulfuric acid on strong-base ion exchangers:Mechanistic insights and modeling[J]. Journal of Chromatography A,2025,1761:466400.
• [33]PERSSON I. Structure and size of complete hydration shells of metal ions and inorganic anions in aqueous solution[J]. Dalton Transactions,2024,53:15517.