刘月娜,程秀红,沈绍传,贠军贤,代斌

• 1:石河子大学化学化工学院
• 2:浙江工业大学化学工程学院绿色化学合成技术国家重点实验室培育基地

摘要(Abstract)：

甘草多糖是甘草提取物中的重要活性组分,具有免疫调节和抗氧化等多种药理作用,对其进行分离纯化的研究具有重要意义。研究探索了一种利用晶胶介质分离甘草多糖的新途径。以预处理和水提甘草根粉末得到的粗多糖为原料,用甲基丙烯酸二甲氨乙酯接枝聚丙烯酰胺晶胶基质制备得到的阴离子交换晶胶介质,对甘草多糖进行了快速层析分离,得到了精制的甘草多糖,确定了适宜的工艺条件。结果表明,阴离子晶胶介质可以吸附甘草多糖,其层析分离迅速;以去离子水为床柱平衡、冲洗及洗脱液配置的基础液,在流速1 cm·min-1条件下,晶胶介质对甘草多糖的吸附容量达670μg·m L-1晶胶,说明晶胶层析可作为天然药用多糖快速分离的一种新方法。

关键词(KeyWords)： 超大孔连续床;晶胶介质;离子交换;分离;甘草多糖

Abstract:

Keywords:

基金项目(Foundation): 科技部中欧政府间国际合作专项项目(1017);; 国家自然科学基金(21036005);; 浙江省自然科学基金(LZ14B060001)

作者(Author): 刘月娜,程秀红,沈绍传,贠军贤,代斌

参考文献(References)：

• [1]Sheela M L,Ramakrishna M K,Salimath B P.Angiogenic and proliferative effects of the cytokine VEGF in Ehrlich ascites tumor cells is inhibited by glycyrrhiza glabra[J].Int Immunopharmacol,2006,6(3):494-498.
• [2]Wan F C,Cheng A W Polysaccharide isolated from glycyrrhiza uralensis Fisch induces intracellular enzyme activity of macrophages[J].Mediterr J Nutr Metab,2009,1(3):165-169.
• [3]Cheng A W,Wan F C,Wang J,et al.Macrophage immunomodulatory activity of polysaccharides isolated from Glycyrrhiza uralensis fish[J].Int Immunopharmacol,2008,8(1):43-50.
• [4]CHENG An-wei(程安玮),JIN Zheng-yu(金征宇),WAN Fa-chun(万发春).Activation effects of glycyrrhiza polysaccharide on mouse peritoneal macrophages.Food Sci(食品科学),2007,28(12):431-434.
• [5]DAI Qiao-yu(代巧玉),KANG Ding(康丁),ZHANG Hong-bin(张洪斌).Development of the isolation,purification,structure and bioactivities of water-soluble glycyrrhiza polysaccharides[J].Nat Prod Res Dev(天然产物研究与开发),2013,25(9):1292-1296.
• [6]Plieva F M,Kirsebom H,Mattiasson B.Preparation of macroporous cryostructurated gel monoliths,their characterization and main applications[J].J Sep Sci,2011,34:2164-2172.
• [7]Kirsebom H,Mattiasson B.Cryostructuration as a tool for preparing highly porous polymer materials[J].Polym Chem,2011,2(5):1059-1062.
• [8]Plieva F M,Galaev I Yu,Noppe W,et al.Cryogel applications in microbiology[J].Trends Microbiol,2008,16(11):543-551.
• [9]Lozinsky V I.Polymeric cryogels as a new family of macroporous and supermacroporous materials for biotechnological purposes[J].Russ Chem Bull,2008,57(5):1015-1032.
• [10]Plieva F M,Galaev I Yu,Mattiasson B.Macroporous gels prepared at subzero temperatures as novel materials for chromatography of particulate-containing fluids and cell culture applications[J].J Sep Sci,2007,30(11):1657-1671.
• [11]Yao K J,Shen S C,Yun J X,at al.Preparation of polyacrylamide-based supermacroporous monolithic cryogel beds under freezing-temperature variation conditions[J].Chem Eng Sci,2006,61(20):6701-6708.
• [12]Yun J X,Shen S C,Chen F,et al.One-step isolation of adenosine triphosphate from crude fermentation broth of Saccharomyces cerevisiae by anion-exchange chromatography using supermacroporous cryogel[J].J Chromatogr B,2007,860(1):57-62.
• [13]Yun J X,Jespersen,G R,Kirsebom H,et al.An improved capillary model for describing the microstructure characteristics,fluid hydrodynamics and breakthrough performance of proteins in cryogel beds[J].J Chromatogr A,2011,1218(32):5487-5497.
• [14]SHEN Zhe-ming(沈哲明),SHEN Shao-chuan(沈绍传),YUN Jun-xian(贠军贤),et al.Purification of adenosine triphosphate from crude fermentation broth by one-step isolation using a large-size weak anion-exchange supermacroporous cryogel(大尺寸弱阴离子交换晶胶层析一步法分离ATP的实验研究)[J].J Chem Eng of Chinese Univ(高校化学工程学报),2009,23(5):765-773.
• [15]He X J,Yao K J,Shen S C,et al.Freezing characteristics of acrylamide-based aqueous solution used for the preparation of supermacroporous cryogels via cryo-copolymerization[J].Chem Eng Sci,2007,62(5):1334-1342.
• [16]Yao K.J,Yun J.X,Shen S.C,et al.In-situ graft-polymerization preparation of cation-exchange supermacroporous cryogel with sulfo groups in glass columns[J].J Chromatogr A,2007,1157(1-2):246-251.
• [17]Yao K.J,Yun J.X,Shen S.C,et al.Characterization of a novel continuous supermacroporous monolithic cryogel embedded with nanoparticles for protein chromatography[J].J Chromatogr A,2006,1109(1):103-110.
• [18]ZHANG Wei-jie(张惟杰).Biochemistry research of glycoconjugates(糖复合物生化研究技术)[M].Hangzhou(杭州):Zhejiang University Press(浙江大学出版社),1999.
• [19]MIAO Jian(缪建),YANG Wen-ge(杨文革),ZHOU Bin(周彬),et al.Extraction and isolation of Lentinan from of lentinus edodes(香菇多糖提取分离的研究)[J].Chinese J Bioproc Eng(生物加工过程),2007,5(3):74-77.
• [20]SUN Ping(孙平),WANG Hai-bo(王海波),CHEN Yuan-yuan(陈媛媛),et al.Study on monosaccharide composition of water-soluble polysaccharide POPS-B1 from Polygonatum Odoratum(玉竹水溶性多糖POPS-B1单糖组成研究[J].China Food Additives(中国食品添加剂),2010(6):166-168.