徐佩伦,乐绍明,张浩哲,钱薇,吴超,李锋,李素静,郭天蛟,李伟,王向前

• 1:浙江大学化学工程与生物工程学院生物质化工教育部重点实验室
• 2:浙江新安江集团股份有限公司
• 3:杭州博盛环保科技有限公司
• 4:杭州澳赛诺生物科技有限公司

摘要(Abstract)：

针对石油化工行业排放的恶臭硫醇和苯、甲苯、乙苯及二甲苯(benzene, toluene, ethyl benzene and xylenen, BTEX)污染物,采用生物法对其进行去除。在生物滴滤塔(biotrickling filter, BTF)中接种二甲苯降解菌Pandoraea sp. WL1和硫醇降解菌Pseudomonas sp. WL2,对硫醇和BTEX的去除性能、动力学特性及微生物群落特性进行详细研究。运行结果表明,优选菌的接种能大大缩短BTF的启动时间。宏观动力学分析表明,该体系混合VOCs的最大去除能力为210.7g×m~(-3)×h~(-1)。16S rRNA测序表明,在经过长时间混合底物的通入后,Hydrogenophaga (17.2%)和Pseudomonas (8.1%)取代Pandoraea成为BTF内的主导菌属。Thiobacillus (4.5%)和Pseudomonas (8.1%)菌属在恶臭硫醇的生物降解中起关键作用。

关键词(KeyWords)： 硫醇;BTEX;生物滴滤塔;微生物群落;16S rRNA测序

Abstract:

Keywords:

基金项目(Foundation): 浙江省科技项目(2017C03007);; 国家重点研发计划(2017YFC0210904)

作者(Author): 徐佩伦,乐绍明,张浩哲,钱薇,吴超,李锋,李素静,郭天蛟,李伟,王向前

参考文献(References)：

• [1] WANG Q L, LI S J, DONG M L, et al. Vocs emission characteristics and priority control analysis based on VOCs emission inventories and ozone formation potentials in Zhoushan[J]. Atmospheric Environment, 2018, 182:234-241.
• [2] ESTRADA J M, KRAAKMAN N J R B, MU OZ R, et al. A comparative analysis of odour treatment technologies in wastewater treatment plants[J]. Environmental Science&Technology, 2011, 45(3):1100-1106.
• [3] MUDLIAR S, GIRI B, PADOLEY K, et al. Bioreactors for treatment of VOCs and odours-A review[J]. Journal of Environmental Management, 2010, 91(5):1039-1054.
• [4] PALAU J, IZQUIERDO M, MARZAL P, et al. Coupling adsorption and biological technologies for multicomponent and fluctuating volatile organic compounds emissions abatement:Laboratory-scale evaluation and full-scale implementation[J]. Industrial&Engineering Chemistry Research, 2015, 54(6):1713-1722.
• [5] VAN GROENESTIJN J W, LAKE M E. Elimination of alkanes from off-gases using biotrickling filters containing two liquid phases[J]. Environmental Progress, 1999, 18(3):151-155.
• [6] GIRI B S, MUDLIAR S N, DESHMUKH S C, et al. Treatment of waste gas containing low concentration of dimethyl sulphide(DMS)in a bench-scale biofilter[J]. Bioresource Technology, 2010, 101(7):2185-2190.
• [7] KENNES C, RENE E R, VEIGA M C. Bioprocesses for air pollution control[J]. Journal of Chemical Technology and Biotechnology, 2009, 84(10):1419-1436.
• [8] MOON C, LEE E Y, PARK S. Biodegradation of gas-phase styrene in a high-performance biotrickling filter using porous polyurethane foam as a packing medium[J]. Biotechnology and Bioprocess Engineering, 2010, 15(3):512-519.
• [9] P REZ M C,áLVAREZ-HORNOS F J, SAN-VALERO P, et al. Microbial community analysis in biotrickling filters treating isopropanol air emissions[J]. Environmental Technology, 2013, 34(19):2789-2798.
• [10] CACERES M, MORALES M, MARTIN R S, et al. Oxidation of volatile reduced sulphur compounds in biotrickling filter inoculated with thiobacillus thioparus[J]. Electronic Journal of Biotechnology, 2010, 13(5):11-12.
• [11] HU J, ZHANG L L, CHEN J M, et al. Performance and microbial analysis of a biotrickling filter inoculated by a specific bacteria consortium for removal of a simulated mixture of pharmaceutical volatile organic compounds[J]. Chemical Engineering Journal,2016, 304:757-765.
• [12] LI H, ZHANG Q, WANG X L, et al. Biodegradation of benzene homologues in contaminated sediment of the East China Sea[J].Bioresource Technology, 2012, 124:129-136.
• [13] WAN S G, LI G Y, AN T C, et al. Biodegradation of ethanethiol in aqueous medium by a new lysinibacillus sphaericus strain rg-1isolated from activated sludge[J]. Biodegradation, 2010, 21(6):1057-1066.
• [14] WANG X Q, WANG Q L, LI S J, et al. Degradation pathway and kinetic analysis for p-xylene removal by a novel pandoraea sp strain wl1 and its application in a biotrickling filter[J]. Journal of Hazardous Materials, 2015, 288:17-24.
• [15] WANG X Q, WU C, LIU N, et al. Degradation of ethyl mercaptan and its major intermediate diethyl disulfide by pseudomonas sp strain wl2[J]. Applied Microbiology and Biotechnology, 2015, 99(7):3211-3220.
• [16] LITTLEJOHNS J V, DAUGULIS A J. Kinetics and interactions of btex compounds during degradation by a bacterial consortium[J].Process Biochemistry, 2008, 43(10):1068-1076.
• [17] MATHUR A K, MAJUMDER C B. Biofiltration and kinetic aspects of a biotrickling filter for the removal of paint solvent mixture laden air stream[J]. Journal of Hazardous Materials, 2008, 152(3):1027-1036.
• [18] SOLOGAR V S, LU Z J, ALLEN D G. Biofiltration of concentrated mixtures of hydrogen sulfide and methanol[J]. Environmental Progress, 2003, 22(2):129-136.
• [19] SU Y, XIA F F, TIAN B H, et al. Microbial community and function of enrichment cultures with methane and toluene[J]. Applied Microbiology And Biotechnology, 2014, 98(7):3121-3131.
• [20] YANG C P, YU G L, ZENG G M, et al. Performance of biotrickling filters packed with structured or cubic polyurethane sponges for VOC removal[J]. Journal of Environmental Sciences, 2011, 23(8):1325-1333.
• [21] SERCU B, N EZ D, AROCA G, et al. Inoculation and start-up of a biotricking filter removing dimethyl sulfide[J]. Chemical Engineering Journal, 2005, 113(2):127-134.
• [22] WAN S G, LI G Y, AN T C, et al. Co-treatment of single, binary and ternary mixture gas of ethanethiol, dimethyl disulfide and thioanisole in a biotrickling filter seeded with lysinibacillus sphaericus rg-1[J]. Journal of Hazardous Materials, 2011, 186(2/3):1050-1057.
• [23] JIME NEZ L, ARRIAGA S, AIZPURU A. Assessing biofiltration repeatability:Statistical comparison of two identical toluene removal systems[J]. Environmental Technology, 2016, 37(6):681-693.
• [24] GALLASTEGUI G,áVALOS RAMIREZ A, EL AS A, et al. Performance and macrokinetic analysis of biofiltration of toluene and p-xylene mixtures in a conventional biofilter packed with inert material[J]. Bioresource Technology, 2011, 102(17):7657-7665.
• [25] HAQUE F, DE VISSCHER A, SEN A. Biofiltration for btex removal[J]. Critical Reviews in Environmental Science and Technology, 2012, 42(24):2648-2692.
• [26] ROY S, GENDRON J, DELHOM NIE M C, et al. Pseudomonas putida as the dominant toluene-degrading bacterial species during air decontamination by biofiltration[J]. Applied Microbiology and Biotechnology, 2003, 61(4):366-373.
• [27] JECHALKE S, FRANCHINI A G, BASTIDA F, et al. Analysis of structure, function, and activity of a benzene-degrading microbial community[J]. Fems Microbiology Ecology, 2013, 85(1):14-26.
• [28] PORTUNE K J, P REZ M C,áLVAREZ-HORNOS F J, et al. Investigating bacterial populations in styrene-degrading biofilters by16s rdna tag pyrosequencing[J]. Applied Microbiology and Biotechnology, 2015, 99(1):3-18
• [29] GRAFF A, STUBNER S. Isolation and molecular characterization of thiosulfate-oxidizing bacteria from an italian rice field soil[J].Systematic and Applied Microbiology, 2003, 26(3):445-452.
• [30] FAHY A, BALL A S, LETHBRIDGE G, et al. Isolation of alkali-tolerant benzene-degrading bacteria from a contaminated aquifer[J].Letters in Applied Microbiology, 2008, 47(1):60-66.
• [31] FERN NDEZ H T, RICO I R, DE LA PRIDA J J, et al. Dimethyl sulfide biofiltration using immobilized hyphomicrobium vs and thiobacillus thioparus tk-m in sugarcane bagasse[J]. Environmental Technology, 2013, 34(2):257-262.
• [32] CHUNG Y C, CHENG C Y, CHEN T Y, et al. Structure of the bacterial community in a biofilter during dimethyl sulfide(DMS)removal processes[J]. Bioresource Technology, 2010, 101(18):7165-7168.
• [33] SEDIGHI M, VAHABZADEH F, ZAMIR S M, et al. Ethanethiol degradation by ralstonia eutropha[J]. Biotechnology and Bioprocess Engineering, 2013, 18(4):827-833.
• [34] MOHAMMAD B T, VEIGA M C, KENNES C. Mesophilic and thermophilic biotreatment of btex-polluted air in reactors[J].Biotechnology and Bioengineering, 2007, 97(6):1423-1438.
• [35] RYU H W, KIM S J, CHO K S, et al. Toluene degradation in a polyurethane biofilter at high loading[J]. Biotechnology and Bioprocess Engineering, 2008, 13(3):360-365.
• [36] LI G Y, HE Z, AN T C, et al. Comparative study of the elimination of toluene vapours in twin biotrickling filters using two microorganisms bacillus cereus s1 and s2[J]. Journal of Chemical Technology and Biotechnology, 2008, 83(7):1019-1026.
• [37] FU B, LIAO X Y, LIANG R, et al. Cod removal from expanded granular sludge bed effluent using a moving bed biofilm reactor and their microbial community analysis[J]. World Journal of Microbiology&Biotechnology, 2011, 27(4):915-923.
• [38] KHAN M I, YANG J, YOO B, et al. Improved rdx detoxification with starch addition using a novel nitrogen-fixing aerobic microbial consortium from soil contaminated with explosives[J]. Journal of Hazardous Materials, 2015, 287:243-251.
• [39] LIU H Z, YAN J P, WANG Q, et al. Biodegradation of methyl tert-butyl ether by enriched bacterial culture[J]. Current Microbiology, 2009, 59(1):30-34.
• [40] ZHANG S Y, WANG Q F, WAN R, et al. Changes in bacterial community of anthracene bioremediation in municipal solid waste composting soil[J]. Journal of Zhejiang University-Science B, 2011, 12(9):760-768.
• [41] LIU T, MAO Y J, SHI Y P, et al. Start-up and bacterial community compositions of partial nitrification in moving bed biofilm reactor[J]. Applied Microbiology and Biotechnology, 2017, 101(6):2563-2574.