朱艳霞,刘天庆,宋克东,马学虎,崔占峰

• 1:大连理工大学化工学院干细胞与组织工程研发中心
• 2:牛津大学工程科学系组织工程与生物处理中心

摘要(Abstract)：

研究表明脂肪组织是多潜能干细胞的又一新来源,脂肪组织来源的干细胞可被用于细胞治疗及组织工程。然而,传统的培养方法很难满足临床需求。为获得大量的脂肪干细胞以满足临床需求,现将脂肪干细胞接种到胶原/壳聚糖支架上,比较细胞在静态环境和在转瓶中动态扩增的情况。通过CCK-8检测细胞增殖情况;并分析葡萄糖和乳酸的代谢情况;14d后扫描电镜观察细胞在支架内的生长情况;流式细胞仪检测干细胞相关表面标记表达;RT-PCR检测干细胞相关转录因子的表达;并检测扩增后的干细胞的多向分化潜能。结果表明:在动态微环境中扩增14d后,与静态条件下相比,支架内的细胞具有更强的增殖活性和更好的多向分化潜能。所扩增的细胞能够保持原有干细胞的特性。结论:所设计的支架-转瓶培养系统是一个简便有效的扩增脂肪干细胞的方法。

关键词(KeyWords)： 脂肪干细胞;扩增;支架;转瓶;组织工程

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30670525);; 大连理工大学青年教师培养基金资助项目(893228)

作者(Author): 朱艳霞,刘天庆,宋克东,马学虎,崔占峰

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