李穗敏,杨谦,赵闯,赵俊,叶勇

• 1:华南理工大学化学与化工学院制药工程系

摘要(Abstract)：

为了提高茶皂苷元的稳定性和抗耐药菌活性,采用复乳法制备光响应茶皂苷元纳米囊。以大豆卵磷脂、胆固醇和脱镁叶绿酸(质量比为50:10:1)为囊材,制得茶皂苷元纳米囊的包封率、载药量、平均粒径、Zeta电位分别为:87.3%±4.6%、13.5%±1.2%、(155±5)nm、(-43.3±2.0)m V。该纳米囊在无光照下具有缓释作用,而在650 nm光照下可加速释放。对耐药金黄色葡萄球菌和大肠杆菌的50%抑菌浓度分别是52.5和85.4μg?m L-1,而与庆大霉素(10μg?m L-1)联合用药时,降至22.6和45.7μg?m L-1,表明它可降低并逆转细菌对庆大霉素的耐药性。分子对接显示茶皂苷元和细菌的甘露醇脱氢酶和黏肽合成酶有显著相互作用,与其抗耐药菌机制相关。

关键词(KeyWords)： 茶皂苷元;纳米囊;光响应;分子对接;抗菌活性;耐药性

Abstract:

Keywords:

基金项目(Foundation): 广州市科技计划项目(1563000123);; 广东省部产学研项目(2016B090918086)

作者(Author): 李穗敏,杨谦,赵闯,赵俊,叶勇

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