吕丹,杜伟,刘德华

• 1:清华大学化学工程系

摘要(Abstract)：

与固定化脂肪酶相比,游离脂肪酶具有反应速率快、成本较低的优势,成为制备生物柴油新的研究方向。前期研究结果表明,游离脂肪酶NS81006可以高效催化大豆油甲醇解制备生物柴油,进一步研究其对含酸油脂的催化,对于促进游离脂肪酶在生物柴油领域中的应用具有重要意义。本文系统研究了甲醇添加策略对游离脂肪酶NS81006催化油酸制备生物柴油的影响,进而考察了NS81006催化模拟酸化油以及实际含酸油脂制备生物柴油的转化情况。研究表明,在优化的甲醇添加策略下,游离脂肪酶NS81006可有效催化油酸、不同含酸量(0~100%,基于总质量)模拟酸化油以及实际含酸油脂进行生物柴油的制备;离心分离可有效实现NS81006的回复使用,连续回用5个批次,游离脂肪酶活性未出现明显下降。

关键词(KeyWords)： 生物柴油;游离脂肪酶;油酸;模拟酸化油;含酸油脂

Abstract:

Keywords:

基金项目(Foundation): 国家863高技术研究发展计划项目(2007AA100703);; 清华大学基础研究基金(JC2007032);; 国家自然科学基金(NSFC20706034)

作者(Author): 吕丹,杜伟,刘德华

参考文献(References)：

• [1]Gerpen J V.Biodiesel processing and production[J].Fuel Process Technol,2005,86(10):1097-1107.
• [2]Li L L,Du W,Liu D H et al.Lipase-catalyzed transesterification of rapeseed oils for biodiesel production with a novel organic solvent as the reaction medium[J].J Mol Catal B:Enzym,2006,43(1-4):58-62.
• [3]Noureddini H,Gao X,Philkana R S.Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil[J].Bioresour Technol,2005,96(7):769-777.
• [4]Oda M,Kaieda M,Hama S et al.Facilitatory effect of immobilized lipase-producing Rhizopus oryzae cells on acyl migration in biodiesel-fuel production[J].Biochem Eng J,2005,23(1):45-51.
• [5]LI Wei(李伟),DU Wei(杜伟),LIU De-hua(刘德华)et al.Intracellular lipase production by immobilized R.orgzae cell and its utilizing for whole cell catalyzed biodiesel production(固定化R.orgzae细胞发酵产胞内脂肪及其催化制备生物柴油)[J].J Chem Eng of Chinese Univ(高校化学工程学报),2008,22(5):822-827.
• [6]Chen X,Du W,Liu D H et al.Lipase-mediated methanolysis of soybean oils for biodiesel production[J].J Chem Technol Biotechnol,2008,83(1):71-76.
• [7]Chen X,Du W,Liu D H.Effect of several factors on soluble lipase-mediated biodiesel preparation in the biphasic aqueous-oil systems[J].World J Microbiol Biotechnol,2008,24(10):2097-2102.
• [8]Chen X,Du W,Liu D H.Response surface optimization of biocatalytic biodiesel production with acid oil[J].Biochem Eng J,2008,40(3):423-429.
• [9]ZHOU Liang-hong(周良虹),HUANG Ya-jing(黄亚晶).Status of biodiesel industry and utilization in overseas countries(国外生物柴油产业与应用状况)[J].Renewable Energy(可再生能源),2005,(4):62-67.
• [10]Felizardo P,Correia M J N,Raposo I et al.Production of biodiesel from waste frying oil[J].Waste Manage,2006,26(5):487-494.
• [11]Haas M J.Improving the economics of biodiesel production through the use of low value lipids as feedstocks:vegetable oil soapstock[J].Fuel Process Technol,2005,86(10):1087-1096.
• [12]Watanabe Y,Shimada Y,Sugihara A et al.Continuous production of biodiesel fuel from vegetable oil using immobilized Candida antarctica lipase[J].J Am Oil Chem Soc,2000,77(4):355-360.
• [13]Shimada Y,Watanabe Y,Samukawa T et al.Conversion of vegetable oil to biodiesel using immobilized Candida antarctica lipase[J].J Am Oil Chem Soc,1999,76(7):789-793.
• [14]Du W,Liu D H,Li L L et al.Mechanism exploration during lipase-mediated methanolysis of renewable oils for biodiesel production in a tert-butanol system[J].Biotechnol Progr,2007,23(5):1087-1090.
• [15]Kazuhiro B,Shinji H,Keiko N et al.Repeated use of whole-cell biocatalysts immobilized within biomass support particles for biodiesel fuel production[J].J Mol Catal B:Enzym,2002,17(3-5):157-65.