廖文竹^1,2,3,4,康淑荷^1,2,3,4,李丽^1,2,3,4,原梦瑶^1,2,3,4,安丽丽^1,2,3,4

• 1:西北民族大学 化工学院， 甘肃 兰州 730106
• 2:环境友好复合材料国家民委重点实验室， 甘肃， 兰州 730106
• 3:甘肃省生物质功能复合材料工程研究中心， 甘肃 兰州 730106
• 4:甘肃省高校环境友好复合材料及生物质利用省级重点实验室， 甘肃 兰州 730106

摘要(Abstract)：

采用响应面法优化大孔吸附树脂纯化兰州百合粗多糖的工艺条件,评估粗多糖和纯化后多糖的美白、保湿和吸湿活性。通过静态实验,筛选出DM301为较佳树脂,在动态实验的基础上,以加权综合评分(包括脱色率、蛋白脱除率和多糖保留率)为响应值,采用响应面法优化DM301纯化百合多糖的较佳工艺条件。以多糖对单酚酶和二酚酶的抑制评价美白活性;考察温度为22℃,相对湿度为11%、43%、81%和95%的环境下24 h内样品的保湿和吸湿活性。在上样质量浓度为4.30 mg·mL^-1、洗脱剂体积为0.70 BV、流速为1.36 BV·h^-1、上样量为1.0 BV条件下,综合评分达63.99%±0.02%(n=3),接近预测值,说明所建立数学模型合理。纯化后多糖对酪氨酸酶单酚酶和二酚酶的抑制率IC₅₀值分别为3.52 mg·mL^-1和2.79 mg·mL^-1,具有良好的美白活性;在22℃,4种湿度条件下,粗多糖和纯化多糖均具有优异的吸湿性能(24 h吸湿率均大于100%);纯化多糖及其混合物均具有高保湿性(24 h保湿率均大于97.5%)。响应面法优化大孔吸附树脂纯化百合粗多糖工艺稳定、可行,为百合多糖的有效纯化提供了理论依据;纯化后的百合多糖的美白、保湿和吸湿活性均有所增强,为兰州百合多糖的开发提供实验基础。

关键词(KeyWords)： 兰州百合多糖;大孔吸附树脂;响应面法;美白活性;吸湿保湿

Abstract:

Keywords:

基金项目(Foundation): 甘肃省教育厅高校教师创新基金(2025B-036);国家自然科学基金(21865028);2024西北民族大学化学创新团队基金(1001660139,1001660141)。

作者(Author): 廖文竹^1,2,3,4,康淑荷^1,2,3,4,李丽^1,2,3,4,原梦瑶^1,2,3,4,安丽丽^1,2,3,4

参考文献(References)：

• [1] 国家药典委员会. 中华人民共和国药典[M]. 北京: 中国医药科技出版社, 2020: 202. Chinese Pharmacopoeia Commission.People's Republic of China pharmacopoeia[M]. Beijing: China Medical Science Press, 2020: 202.
  [2] TANG Y C, LIU Y J, HE G R, et al. Comprehensive analysis of secondary metabolites in the extracts from different lily bulbs and their antioxidant ability[J].Antioxidants, 2021, 10(10): 1634.
  [3] GUO J W, LU L N, LI J, et al. Extraction, structure, pharmacological activity, and structural modification of Lilium polysaccharides[J].Fitoterapia, 2024, 172: 105760.
  [4] KANG S, GUO Z, ZHAO F, et al. Lanzhou Lily polysaccharide fragment protects human umbilical vein endothelial cells from radiation-induced DNA double-strand breaks[J].Human & Experimental Toxicology, 2022, 41: 9603271221140110.
  [5] 王昭珺, 刘晓风, 徐也. 兰州百合芯多糖的提取工艺及其体外抗氧化活性[J]. 现代食品科技, 2020, 36(9): 219-227, 201. WANG Z J, LIU X F, XU Y. Extraction process of polysaccharides from Lanzhou lily cores and evaluation of their antioxidant activity in vitro[J].Modern Food Science and Technology, 2020, 36(9): 219-227, 201.
  [6] 李刚刚, 孙静, 伏衡一, 等. 兰州百合鳞茎多糖超声波辅助提取工艺优化及稳定性研究[J]. 食品与机械, 2020, 36(3): 190-193. LI G G, SUN J, FU H Y, et al. Research on ultrasonic-assisted extraction process and stability of polysaccharides from Lilium davidii var bulb[J].Food & Machinery, 2020, 36(3): 190-193.
  [7] 孙国威, 米雪, 周彦军, 等. 百合多糖提取及其止咳作用研究[J]. 农产品加工, 2020(20): 8-11, 14. SUN G W, MI X, ZHOU Y J, et al. Studies on extraction and antitussive effect of lily polysaccharide[J].Farm Products Processing, 2020(20): 8-11, 14.
  [8] 周静华, 万顺康. 大理百合多糖的醇沉分离工艺及脱蛋白方法[J]. 食品研究与开发, 2010, 31(12): 7-9. ZHOU J H, WAN S K. Study on ethanol precipitation process and deproteinization in extraction of polysaccharides from DaLi Lilium brownie[J].Food Research and Development, 2010, 31(12): 7-9.
  [9] LIU H, YANG S J, REN H K, et al. Research progress of extraction and isolation of natural plant polysaccharides and their applications in skin care[J].China Surfactant Detergent & Cosmetics, 2024, 54(6): 708-714.
  [10] 闫昱辛, 何美军, 李宇, 等. 铁皮石斛叶多糖美白和抗氧化活性[J]. 植物研究, 2025, 45(1): 111-118. YAN Y X, HE M J, LI Y, et al. Whitening and Antioxidant Activities of Leaf Polysaccharide from Dendrobium officinale[J].Bulletin of Botanical Research, 2025, 45(1): 111-118.
  [11] 李思宇. 米邦塔仙人掌多糖的美白保湿功效研究及产品开发[D]. 贵州: 贵州师范大学, 2024: 46-56. LI S Y. Study on whitening and moisturizing effect of polysaccharide from Opuntia Milpa Alta and product development[D]. Guizhou: Guizhou Normal University, 2024: 46-56.
  [12] 詹梦茹, 王彦斌, 康淑荷, 等. 党参多糖的大孔树脂纯化及其美白、吸湿保湿性能[J]. 林产化学与工业, 2023, 43(3): 79-88. ZHAN M R, WANG Y B, KANG S H, et al. Purification of polysaccharides from Codonopsis pilosula by macroporous adsorption resin and its whitening, moisture absorption and moisturizing properties[J].Chemistry and Industry of Forest Products, 2023, 43(3): 79-88.
  [13] 何林蔓, 柳宜池, 陈莎, 等. 血耳伴生菌胞外多糖的抗氧化活性及吸湿、保湿性能[J]. 食品研究与开发, 2023, 44(17): 30-36. HE L M, LIU Y C, CHEN S, et al. Antioxidant activity, moisture-absorption and moisture-retention properties of exopolysaccharides from the associated fungus of Tremella sanguinea[J].Food Research and Development, 2023, 44(17): 30-36.
  [14] 邓演, 赵得志, 冉金红, 等. 超声波辅助热水浸提法提取兰州百合多糖的工艺研究[J]. 化工管理, 2016(11): 216. DENG Y, ZHAO D Z, RAN J H, et al. Study on ultrasonic-assisted extraction of polysaccharide from Lanzhou lily thunb[J].Chemical Engineering Management, 2016(11): 216.
  [15] 陈政, 高友君, 邓芳飞, 等. 微波提取桂花叶多糖及其吸湿保湿性和抗氧化性研究[J]. 化工新型材料, 2022, 50(增刊1): 337-340. CHEN Z, GAO Y J, DENG F F, et al. Microwave extraction of osmanthus leaf polysaccharide and its moisture-absorption and moisture-retention and antioxidant properties[J].New Chemical Materials, 2022, 50(Suppl.1): 337-340.
  [16] 田艳花, 杨兆艳, 罗爱国, 等. 微波辅助双水相提取百合多糖的工艺优化及其结构表征[J]. 食品工业科技, 2023, 44(21): 227-233. TIAN Y H, YANG Z Y, LUO A G, et al. Optimization of microwave assisted aqueous two phase extraction of lily polysaccharides and its structure characterization[J].Science and Technology of Food Industry, 2023, 44(21): 227-233.
  [17] 侯彦辉, 李喜阁, 张凤仪, 等. 银耳多糖的纯化及其生物活性[J]. 天津工业大学学报, 2024, 43(2): 60-67, 74. HOU Y H, LI X G, ZHANG F Y, et al. Purification and biological activity of Tremella fuciformis polysaccharides[J].Journal of Tiangong University, 2024, 43(2): 60-67, 74.
  [18] 周锦燕, 张芸, 刘良燕, 等. 刺五加新品种'紫加1号’多糖分离纯化及抗氧化活性分析[J]. 广西植物, 2024, 44(2): 362-372. ZHOU J Y, ZHANG Y, LIU L Y, et al. Isolation and purification the polysaccharides and its antioxidant activity of 'Zijia 1’ novel variety of Acanthopanax senticosus[J].Guihaia, 2024, 44(2): 362-372.
  [19] 吴霞, 周攀, 常雪婷, 等. 响应面法优化羊栖菜多糖脱蛋白工艺及其抗氧化活性分析[J]. 粮食与油脂, 2024, 37(12): 135-141. WU X, ZHOU P, CHANG X T, et al. Optimization of deproteinization process of Sargassum fusiforme polysaccharide by response surface methodology and analysis of its antioxidant activity[J].Cereals & Oils, 2024, 37(12): 135-141.
  [20] 潘峰, 阮国永, 杨建波, 等. 大孔吸附树脂纯化吴茱萸果实多糖工艺优化[J]. 现代食品科技, 2021, 37(9): 216-225, 304. PAN F, RUAN G Y, YANG J B, et al. Optimization of the purification process for the polysaccharides from Tetradium ruticarpum fruit using macroporous adsorption resins[J].Modern Food Science and Technology, 2021, 37(9): 216-225, 304.
  [21] 刘冲英, 周宁, 崔涛, 等. 响应面法优化大孔树脂纯化地黄多糖工艺[J]. 食品工业科技, 2021, 42(6): 202-207, 232. LIU C Y, ZHOU N, CUI T, et al. Optimization of purification process of polysaccharides from Rehmannia glutinosa Libosch. with macroporous adsorption resins by response surface methodology[J].Science and Technology of Food Industry, 2021, 42(6): 202-207,232.
  [22] 王红. 银柴胡多糖的提取、纯化、鉴定及对肠道微生物的影响研究[D]. 银川: 宁夏大学, 2023: 1-56. WANG H. Study on extraction, purification, identification of polysaccharide from Stellariae Radix (Yin Chai Hu) and effect on intestinal microorganisms[D]. Yinchuan: Ningxia University, 2023: 1-56.
  [23] MOORTHY A, GOVINDAN S, JANARDHANAN A, et al. Response surface methodology optimization extraction of polysaccharide from Tricholoma lobayense and its biological activities[J].Journal of Food Measurement And Characterization, 2024, 18(3): 2133-2145.
  [24] 涂玲飞, 李焱, 张振. 响应面优化硒化白及多糖的制备工艺及其体外抗氧化活性研究[J]. 食品工业科技, 2024, 45(7): 244-253. XU L F, LI Y, ZHANG Z. Optimization of Selenized Bletilla striata polysaccharides preparation by response surface methodology and determination of the antioxidant activity in vitro[J].Science and Technology of Food Industry, 2024, 45(7): 244-253.
  [25] 李海馨, 何婧芝, 冯文亚, 等. 白芷美白活性部位初步研究[J]. 天然产物研究与开发, 2018, 30(3): 425-428, 514. LI H X, HE J Z, FENG W Y, et al. Preliminary studies on the active fraction responsible for whitening activity of Angelicae dahuricae[J].Natural Product Research and Development, 2018, 30(3): 425-428, 514.
  [26] 张玩涛, 程茜菲, 彭修娟, 等. 百合多糖提取工艺优化及吸湿保湿性能研究[J]. 化学与生物工程, 2024, 41(8): 38-44, 68. ZHANG W T, CHENG Q F, PENG X J, et al. Optimization in extraction process of polysaccharide from Lilium brownii and its moisture-absorption and moisture-retention properties[J].Chemistry &Bioengineering, 2024, 41(8): 38-44, 68.
  [27] 蒋建新, 张乐平, 徐伟, 等. 特色高分子多糖改性及应用研究进展[J]. 林业工程学报, 2022, 7(6): 13-24. JANG J X, ZHANG L P, XU W, et al. Advance on modification and application of characteristic macromolecular polysaccharides[J].Journal of Forestry Engineering, 2022, 7(6): 13-24.
  [28] 何晓梅, 赵良冰, 金新茹, 等. 大别山白及多糖制备及其吸湿保湿性能研究[J]. 中国野生植物资源, 2023, 42(6): 37-40, 52. HE X M, ZHAO L B, JIN X R, et al. Study on the preparation, moisture absorption and retention of polysaccharides of Bletilla striata (Thunb.) Rchb. F. in Dabie Mountain area[J].Chinese Wild Plant Resources, 2023, 42(6): 37-40, 52.